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Transplantation of mesenchymal stem cells enhances axonal outgrowth and cell survival in an organotypic spinal cord slice culture

Cited 34 time in Web of Science Cited 41 time in Scopus
Authors
Cho, Jung-Sun; Park, Hwan-Woo; Park, Sang-Kyu; Roh, Sangho; Kang, Soo-Kyung; Paik, Ki-Suk; Chang, Mi-Sook
Issue Date
2009-04
Publisher
Elsevier
Citation
Neuroscience Letters 454 (2009) 43-48
Keywords
Organotypic slice cultureDemyelinationMesenchymal stem cellsAxonal outgrowthSpinal cord injury
Abstract
Mesenchymal stem cells (MSCs) have demonstrated a measurable therapeutic effect following transplantation into animal models of spinal cord injury. However, the mechanism(s) by which transplanted cells promote nerve regeneration and/or functional recovery remains indeterminate. Several studies have suggested that MSCs promote tissue repair via secretion of trophic factors, but delineating the effect of such factors is difficult due to the complexity of the in vivo systems. Therefore, we developed an organotypic spinal cord slice culture system that can be sustained for sufficient periods of time in vitro to evaluate nerve regeneration as an ex vivo model of spinal cord injury. Using this model, we demonstrate that treatment of lumbar slices of spinal cord with lysolecithin induced a significant degree of cell death and demyelination of nerve fibers, but that these effects were ameliorated to a significant extent following co-culture of slices with human MSCs (hMSCs). The results indicate that transplanted hMSCs alter the tissue microenvironment in a way that promotes survival of endogenous cells, including injured neurons, immature oligodendrocytes and oligodendrocyte progenitor cells. This ex vivo culture system represents a useful tool to further dissect the mechanism(s) by which MSCs promote regeneration of injured nervous tissue.
ISSN
0304-3940
Language
English
URI
http://hdl.handle.net/10371/69701
DOI
https://doi.org/10.1016/j.neulet.2009.02.024
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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