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Identification and regulation of K+ & Cl- channels in human parotid acinar cells
DC Field | Value | Language |
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dc.contributor.author | Park, Kyungpyo | - |
dc.contributor.author | Case, R. Maynard | - |
dc.contributor.author | Brown, Peter D | - |
dc.date.accessioned | 2010-09-09T06:50:30Z | - |
dc.date.available | 2010-09-09T06:50:30Z | - |
dc.date.issued | 2001-09 | - |
dc.identifier.citation | Archives of Oral Biology. 46, 801-810 | en |
dc.identifier.issn | 0003-9969 | - |
dc.identifier.uri | https://hdl.handle.net/10371/69791 | - |
dc.description.abstract | The properties of K+ channels in these cells were studied using patch-clamp methods. Two channels, with conductances of 165±13 pS (n=6) and 30±1 pS (n=3), were identified in single-channel experiments. In cell-attached patches the reversal potentials were −67±8 and −74±2 mV for the large and small conductance channel, respectively, suggesting that both channels are K+-selective. The large conductance channel was also shown to be K+-selective in inside-out patches. The open probability (Po) of this channel was increased at depolarizing potentials and by increasing intracellular Ca2+ concentration ([Ca2+]i). These properties suggest that the large conductance channel is a maxi Ca2+-activated K+ channel (BKCa). The small conductance channel was not observed in inside-out patches. Carbachol (CCh; 10−5 M) activated the BKCa channel, but not the small conductance channel, in cell-attached patches. CCh also caused a dose-dependent increase in [Ca2+]i measured by fura-2 in microspectrofluorimetric studies, with a half-maximal response at approximately 3×10−6 M. Neither isoproterenol (10−5 M) nor substance P (10−6 M) affected K+-channel activity or [Ca2+]i. In whole-cell experiments, CCh caused an increase in outward current. Charybdotoxin (10−7 M), a BKCa blocker, inhibited a large component of the CCh-induced current. A large component of the charybdotoxin-insensitive current may be carried by Ca2+-activated Cl− channels, which were also observed in human parotid acinar cells. The results indicate that BKCa channels make a significant contribution to the whole-cell conductance in human parotid acinar cells. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.title | Identification and regulation of K+ & Cl- channels in human parotid acinar cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 박경표 | - |
dc.identifier.doi | 10.1016/S0003-9969(01)00047-4 | - |
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