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Analysis of differential gene expression in auricular lymph nodes draining skin exposed to sensitizers and irritants

Cited 0 time in webofscience Cited 9 time in scopus
Authors
Ku, Hyun-Ok; Jeong, Sang-Hee; Kang, Hwan-Goo; Pyo, Hyun-Mi; Cho, Joon-Hyoung; Son, Seong-Wan; Ryu, Doug Young
Issue Date
2007-12-16
Publisher
Elsevier
Citation
Toxicol. Lett. 177, 1–9
Keywords
Murine local lymph node assayMicroarrayIfi27Il12rb1IfngZbp1
Abstract
There has been some concern that certain non-sensitizing irritants may yield false positive results in the murine local lymph node assay (LLNA). This study compared gene expression profiles in lymph nodes draining skin following exposure to sensitizers and irritants, to identify gene transcripts that could distinguish sensitizers from irritants. After treating CBA/N mouse ears for 3 days with the sensitizers 1-chloro-2,4-dinitrobenzene, 2-phenyl-4-ethoxymethylene-5-oxazolone, or toluene-2,4-diisocyanate or the non-sensitizing irritants croton oil or nonanoic acid, auricular lymph nodes and ear tissues were excised. Sensitizer-induced changes in parameters such as ear thickness, lymph node weight, and cell count also occurred in irritant-treated mouse tissues. However, gene transcripts such as Ifi27, Il12rb1, Ifng, and Zbp1, which are related to T-cell activation, were shown by gene expression microarrays and real-time RT-PCR analyses to be up-regulated in auricular lymph nodes by sensitizers exclusively. These findings suggest that gene expression analysis may enable distinction between sensitizing chemicals and non-sensitizing irritants.
ISSN
0378-4274
Language
English
URI
http://hdl.handle.net/10371/7027
DOI
https://doi.org/10.1016/j.toxlet.2007.12.003
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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