Publications
Detailed Information
Phage Therapy against β-hemolytic Streptococcicosis of Japanese Flounder Paralichthys olivaceus : ヒラメのβ溶血性レンサ球菌症に対するファージ治療試験
Cited 23 time in
Web of Science
Cited 23 time in Scopus
- Authors
- Issue Date
- 2007
- Publisher
- Japanese Society of Fish Pathology
- Citation
- Fish Pathol., 42, 181-189
- Keywords
- bacteriophages ; phage therapy ; β-hemolytic streptococcicosis ; Streptococcus iniae ; Paralichthys olivaceus ; Japanese flounder
- Abstract
- We examined the therapeutic effect of Streptococcus iniae phages isolated from fish culture environments against experimental streptococcicosis of Japanese flounder Paralichthys olivaceus. Phage sensitivity tests with a double agar method revealed that 31 of 35 S. iniae strains from the flounder have a similar sensitivity to six phage isolates. In phage therapy experiments, fish were injected intraperitoneally (IP) with S. iniae PSi402 and 1 h later IP-injected with a mixture of two or four phage isolates, and observed at 25°C for 2 wk. Mortalities of fish receiving phages were significantly lower than those of control fish without phage-treatment in all four trials. The effect of phage treatment was also demonstrated even at 24 h post-infection, when cell numbers of S. iniae were 107.4 and 104.5 CFU/g in the kidneys and brains of fish, respectively. However, as phage-resistant S. iniae were frequently isolated from dead fish in the phage-treated group, further investigations are required to establish phage therapy of the disease.
- ISSN
- 0388-788X (print)
1881-7335 (online)
- Language
- Japanese
- Files in This Item:
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.