SHERP

Phage Therapy against β-hemolytic Streptococcicosis of Japanese Flounder Paralichthys olivaceus
ヒラメのβ溶血性レンサ球菌症に対するファージ治療試験

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Authors
Matsuoka, Satoru; Hashizume, Takaya; Kanzaki, Hiroyuki; Iwamoto, Emi; Park, Se Chang; Yoshida, Terutoyo; Nakai, Toshihiro
Issue Date
2007
Publisher
Japanese Society of Fish Pathology
Citation
Fish Pathol., 42, 181-189
Keywords
bacteriophagesphage therapyβ-hemolytic streptococcicosisStreptococcus iniaeParalichthys olivaceusJapanese flounder
Abstract
We examined the therapeutic effect of Streptococcus iniae phages isolated from fish culture environments against experimental streptococcicosis of Japanese flounder Paralichthys olivaceus. Phage sensitivity tests with a double agar method revealed that 31 of 35 S. iniae strains from the flounder have a similar sensitivity to six phage isolates. In phage therapy experiments, fish were injected intraperitoneally (IP) with S. iniae PSi402 and 1 h later IP-injected with a mixture of two or four phage isolates, and observed at 25°C for 2 wk. Mortalities of fish receiving phages were significantly lower than those of control fish without phage-treatment in all four trials. The effect of phage treatment was also demonstrated even at 24 h post-infection, when cell numbers of S. iniae were 107.4 and 104.5 CFU/g in the kidneys and brains of fish, respectively. However, as phage-resistant S. iniae were frequently isolated from dead fish in the phage-treated group, further investigations are required to establish phage therapy of the disease.
ISSN
0388-788X (print)
1881-7335 (online)
Language
Japanese
URI
http://hdl.handle.net/10371/7048
DOI
https://doi.org/10.3147/jsfp.42.181
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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