태생중기태아 선하수체세포에 대한 조직화학반응의 비교관찰

Abstract

Nineteen human fetal hypophyses ranging 5 to 8 months in gestational age were studied h istochemically to observe the changes of stainability. number of cells and distribution of chromophils in adenohypophysis with increasing fetal age. The pituitary glands were excised and fixed in Bouin's fluid. and serial sections were made at 5p.. Tissue sections were stained with Hematoxylin-Eosin. Masson's trichrome. Azocarmine'Aniline Blue-Orange G. PAS-Methyl Blue-Orange G and Aldehyde Fuchsin-PAS-Orange G. The results observed under ordinary light microscope were as follows. 1. Stainability was much lower than adult, but acidophils and basophils were identified and distinguishable from 5 months, and somatotrophs and marnmotrophs were distinguishable from 7 months. 2. Both acidophils and basophils increased in number with advancing fetal age particularly on 8 month, and acidophils exceeded basophils in increasing rate. 3. Acidophils were distributed mainly in the anterior portion of Rathke's pouch, but basophils in the peripheral portion of adenohypophysis.