S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 17 No.4 (1976)
Immunological Studies on the MeA-induced Sarcoma using the MIF Technique
실험적으로 유발한 도오물종양에 있어서 거식세포 유주저지법을 이용한 면역학적 연구
- Shin, Hee-Sup; Rhee, Kwang-Ho; Kim, Ik-Sang; Chang, Woo-Hyun; Lee, Seung-Hoon
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.17 No.4, pp. 383-391
- It has been generally agreed that in a che.
mically induced, tumor the tumor specific antigen
-could be identified by circulating humoral antibody
and transplantation test among inbred ani.
To quantify the cellular immunity to the MCA-
induced tumor and to assess the in vitro effect
-of serum of mice which were bearing advanced
tumors, the authors adopted the MIF test.
And the results obtained are summarized as
1. MIF technique has been established using
the mammalian tumor cells as antigen with
the ratio of peritoneal cells to tumor cells
as 10: 1 or 1: 1, and the test could be
performed with good results by adding the
tumor cells directly into the media of the
chamber instead of mixing with the peritoneal
cells in the capillary tubes.
:2. The antigenicity of the MCA-induced sareorna in C3H/HeN mice was detected by MIF
technique. When peritoneal cells obtained
from mice sensitized with frozen-thawed tumor
cells were challenged with the same tumor
cells in vitro, they were inhibited significantly
from migration on the glass surface as compared
with those from normal mice.
3. In the host which had received viable tumor
cells but not yet developed a grossly visible or
palpable tumor, in vitro cell mediated immune
response to the tumor cells(detected by MIF
test) was the same as that of actively sensitized
4. The blocking effect on the cell mediated
immunity in vitro of the sera obtained from
mice which were bearing advanced tumors
was detected by using the sera as substitute
for normal sera in the media of MIF chambers
and in the procedure of washing the peritoneal
cells before contact with the tumor cells.