녹농균 및 포도구균이 단독 또는 혼재할때의 식균양상에 대한 실험적 관찰

Abstract

Bacterial suspension of Ps. aeruginosa, Staph. aureus and the mixture of the two were added to rabbit macrophage suspended in Cell Maintenance Fluid (CMF) and normal rabbit serum. After an incubation period of 30 and 60 minutes at 37'C, the bacteria-macrophage suspensions were investigated as regard to the percentages of macrophage with intracellular organisms and percentages of intracellular organisms. Results of observation were summarized as follows; 1. In the Ps. aeruginosa-macrophage suspension; The percentages of macrophage with intracellular organisms were 15% after 30 minutes, 29% after 60 minutes in normal rabbit serum, and 11. 5% after 30 minutes, 46% after 60minutes in CMF respectively. Percentages of intracellar organisms were 97% after 30 minutes, 97% after 60 minutes in normal rabbit serum, and 92% after 30 minutes, 96% after 60 minutes in CMF. 2. In the Staph. aureus-rnacrophage suspension; The percentages with intracellular organisms were 45.5% after 30 minutes, 68% after 60 minutes in normal rabbit serum, and 47% after 30 minutes, 80, 5% after 60 minutes in CMF respecitively. Percentages of intractracellular organisms were 91% after 30 minutes, 97. 5% after 60 minutes in normal rabbit serum, and 79.5% after 30 minutes, 89.5% after 60 minutes in CMF. 3. In the bacterial mixture-macrophage suspension; The percentages of macrophage with intracellular organisms were 25.5% after 30 minutes, 67.5% after 60 minutes in normal rabbit serum, and 48.5% after 30 minutes, 82.5% after 60 minutes in CMF respectively. The percentages of intracellular organisms. on the other hand, were 98.5% after 30 minutes, 93% after 60 minutes in normal rabbit serum, and 59% after 30 minutes, 71% after 60 minutes in CMF. Staph. aureus VB. Ps. aeruginosa of intracellular organisms were 69% :31% after 30 minutes, 92%: 8% after 60 minutes in normal rabbit serum, and 62%:38% after 3Ominutes, 83%:17% after 60 minutes in CMF.