S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 31 No.3 (1990)
An Immunocytochemical Study on the Differentiation of Synoviocytes in the Knee Joint of Mouse
생쥐 활막세포의 분화에 관한 면역세포화학적 연구
- Hwang, Douk Ho; Lee, Joong Myung; Chang, Ka Young
- Issue Date
- Seoul J Med, Vol.31 No.3, pp. 155-166
- This study was designed to observe the differentiation of synoviocytes
in the knee joint of mouse by immunocytochemical technique.
The feti were collected on the 15th, 16th, 17th, 18th and 19th day of pregnancy,
and the I-day-old and 5-day-old neonates were also collected.
The knee joints were fixed in Bourn's solution and processed and finally embedded
in paraplast by routine method. The serial sections were stained in H&E.
In order to visualize the lysozyme and the Ia antigen in the cell, rabbit anti-lysozyme
antibody and monoclonal IgG2a anti-mouse I-Ad were used in the double-bridge peroxidase
To observe the ultrastructure of the synoviocytes, the junctional complex, the basal
lamina and the type of capillaries in the synovial membrane, some tissues were embedded
in Epon 812 by the usual method.
The joint cavities were formed separately in the posterior portion of the patella and
between the femur and tibia in the Ifi-day-old fetus. These were united in the 18-dayold
fetus. The synoviocytes, which showed a positive reaction to anti-lysozyme, first
appeared in the 18-day-old fetus. These synoviocytes were abundant on the luminal
side of the primordial infrapatellar fat pad in the 19-day-old fetus but were absent
on the surface of the meniscus and cruciate ligaments.
The Ia antigen-positive synoviocytes first appeared in the 5-day-old neonate. In the
types of synovial membrane, the fibrous type first appeared in the 17-day-old fetus,
the areolar type in the 18-day-old fetus, and the adipose type in the 5-day-old neonate.
The synoviocytes did not have the basal lamina at any age. The desmosomal junctions
were observed between the synoviocytes in the 8-week-old adult.
The capillaries supplying blood in the synovial membrane were the fenestrated type
with closing diaphragms, but the basal lamina was incomplete.
On the basis of above findings, it is obvious that joint cavities are formed by the
fusion of small ones during fetal growth. The lysozyme-positive synoviocytes, which
compose the synovial membrane, appear before birth, but the expression of the Ia antigen
shows up after birth.