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Generation of Cells With Hodgkin's and Reed-Sternberg Phenotype Through Downregulation of CD99 (Mic2)

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Authors
Kim, Soon Ha; Choi, Eun Young; Shin, Young Kee; Kim, Tae Jin; Chung, Doo Hyun; Chang, Sung Ik; Kim, Noe Kyeong; Park, Seong Hoe
Issue Date
1998-12
Publisher
American Society of Hematology
Citation
Blood; Vol.92, No.11; pp.4287-4295
Abstract
Despite the fact that Hodgkin’s and Reed-Sternberg (H-RS) cells are morphological hallmarks of Hodgkin’s disease (HD), the nature of H-RS cells still remains to be resolved. Here we report that downregulation of CD99 (Mic2) leads to the generation of cells with an H-RS phenotype. IM9 and BJAB B-cell lines that were transfected with an antisense CD99 expression construct showed the morphological and immunological characteristics of H-RS cells such as multinuclearity, expression of CD15, decreased expression of major histocompatibility complex (MHC) class I and CD45RB, and deregulated secretion of cytokines. The reduced expression of CD99 was also confirmed in H-RS cells of patient’s lymph nodes and three HD-derived cell lines, L428, KM-H2, and HDLM-2. Moreover, features characteristic of H-RS cells were completely abolished by forced expression of CD99 and by a constitutively active form of Rac, which functions downstream of CD99. We suggest that CD99 molecules play a crucial role in regulating functions and morphology of cells through a Rac-Rho signaling pathway and that the loss of CD99 expression is a significant molecular event to generate H-RS cells.
ISSN
1528-0020
Language
English
URI
http://hdl.handle.net/10371/73415
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College of Pharmacy (약학대학)Dept. of Pharmacy (약학과)Journal Papers (저널논문_약학과)
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