Publications
Detailed Information
Characteristics of Ca Currents in Rabbit Basilar Arterial Smooth Muscle Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | So, Insuk | - |
dc.contributor.author | Kang, Tong Mook | - |
dc.contributor.author | Kim, Ki Whan | - |
dc.date.accessioned | 2009-08-20T05:35:03Z | - |
dc.date.available | 2009-08-20T05:35:03Z | - |
dc.date.issued | 1994-09 | - |
dc.identifier.citation | Seoul J Med, Vol.35 No.3, pp. 169-182 | - |
dc.identifier.issn | 0582-6802 | - |
dc.identifier.uri | https://hdl.handle.net/10371/7345 | - |
dc.description.abstract | In order to determine the exact nature of Ca channels involved in various
cerebrovascular contractile behaviour including vasospasm, we performed experiments to identify and characterize the types of Ca channels in rabbit basilar arterial smooth muscle cells by using kinetic and pharmacologic tools. Sngle smooth muscle cells were enzymatically isolated from rabbit basilar artery. Single cells were voltageclamped, and membrane currents were recorded using the whole-cell configuration of patch clamp technique. The measured cell capacitance (Cm) was 19.2 ± 0.65 pF ( n 21) and input resistance (Rmpu, ) was 2.04 ± 0.12 Gn(n 12), These passive membame properties are similar to other cerebraovascular smooth muscle cells. Inward Ca2 ' -channel current was recorded. Replacement of external Ca2+( 2 mM) with Ba" (10 mM) increased the amplitude of the current and did not shift the I-V relationship for lBa in comparison with that for Ie.. Changing the holding potential from -SO to -40 mV decreased the current amplitude but did not shift the voltage dependence. No detectable low-threshold, rapid inactivating inward current was observed. Steady-state activation and inactivation curves for lea(V, 210c') -4.4 mV; V, 2(inac,) -22.3 mV) and lBa(v, 2(oc') -7.5 mV; VI 2 {Inact } -20.5 mV) were determined. The theoritical 'window current's amplitude was calculated for lea and lBa• Calcium channel current was almost completely inhibited by l,uM nicardipine and enhanced by Bay K 8644, suggesting this is carried by 'L-type' but not by 'T-type' calcium channel. Bay K 8644 significantly shifted activation curve to the negative potential. Both 8-br-cAMPW.l ~ 1 mM) and 8-br-cGMP( 0.1 ~ 1 mM), a membrane permeable cyclic nucleotides, decreased the current amplitude. From the above results, it is suggested that only 'L-type' Ca-current(ICa_d exists in rabbit basilar arterial smooth muscle cells. | - |
dc.language.iso | en | - |
dc.publisher | Seoul National University College of Medicine | - |
dc.subject | L-type Ca currents | - |
dc.subject | basilar artery | - |
dc.subject | patch clamp | - |
dc.subject | window current | - |
dc.title | Characteristics of Ca Currents in Rabbit Basilar Arterial Smooth Muscle Cells | - |
dc.type | SNU Journal | - |
dc.contributor.AlternativeAuthor | 서인석 | - |
dc.contributor.AlternativeAuthor | 강동묵 | - |
dc.contributor.AlternativeAuthor | 김기환 | - |
dc.citation.journaltitle | 서울 의대 잡지 | - |
dc.citation.journaltitle | 서울 의대 학술지 | - |
dc.citation.journaltitle | Seoul Journal of Medicine | - |
dc.citation.endpage | 182 | - |
dc.citation.number | 3 | - |
dc.citation.pages | 169-182 | - |
dc.citation.startpage | 169 | - |
dc.citation.volume | 35 | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.