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Induction of a Neuronal Phenotype from Human Bone Marrow-Derived Mesenchymal Stem Cells

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Authors
Oh, Soonyi; Park, Hwan-Woo; Cho, Jung-Sun; Jung, Heekyung; Lee, Seung-Pyo; Paik, Ki-Suk; Chang, Mi-Sook
Issue Date
2009-12-31
Publisher
The Korean Academy of Oral Biology
Citation
International Journal of Oral Biology Vol.34 No.4, pp. 177-183
Keywords
mesenchymal stem cellstranscription factorneurondifferentiationinduction
Abstract
Human mesenchymal stem cell (hMSCs) isolated from human adult bone marrow have self-renewal capacity and can differentiate into multiple cell types in vitro and in vivo. A number of studies have now demonstrated that MSCs can differentiate into various neuronal populations. Due to their autologous characteristics, replacement therapy using MSCs is considered to be safe and does not involve immunological complications. The basic helix-loop-helix (bHLH) transcription factor Olig2 is necessary for the specification of both oligodendrocytes and motor neurons during vertebrate embryogenesis. To develop an efficient method for inducing neuronal differentiation from MSCs, we attempted to optimize the culture conditions and combination with Olig2 gene overexpression. We observed neuron-like morphological changes in the hMSCs under these induction conditions and examined neuronal marker expression in these cells by RTPCR and immunocytochemistry. Our data demonstrate that the combination of Olig2 overexpression and neuron-specific conditioned medium facilitates the neuronal differentiation of hMSCs in vitro. These results will advance the development of an efficient stem cell-mediated cell therapy for human neurodegenerative diseases.
ISSN
1226-7155
Language
English
URI
http://hdl.handle.net/10371/73525
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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