Genotyping via Amplification and Hhal Cleavage of Apolipoprotein E (ApoE) Gene in Patients with Coronary Artery Disease

Abstract

DNA amplification and HhaI cleavage for the apolipoprotein E (apofi) genotyping is identified in 108 patients with coronary artery disease (CAD). A group of randomly selected 100 normal individuals analysed as control samples. There were no significant differences in apoE genotype frequencies between the two groups. Analysis of variances was performed to test for mean differences in plasma lipids. lipoprotein and apolipoprotein B (apoB) levels. Compared to controls, patients with CAD had significantly higher levels of total cholesterol (TC) and low density lipoprotein-cholesterol (LDL-ChoI). The ratios of LDL-Chol to apoB were also elevated in the CAD group. The levels of TC and LDL-Chol in the E3j3 genotype of patients were significantly higher than those of the controls. The high density Iipoprotein-cholesteroI(HDL-ChoI) levels in E4j3 type of the patients were significantly higher than those of E3j3 subjects. As phenotype analysis for the apoE polymorphism differs somewhat among populations, so we discussed possible reasons for these discrepancies.