A Study of the in Vitro Effect of L-Asparaginase, EC-l and EC-2, on the Glycine and Nucleic Acid Contents in Regenerating Liver of Albino Rats

Abstract

Two Lvasparagtnases, designated EC-l and EC-2. have been partially purified from E. coli 0112a. and incubated respectively with the homogenates of the regenerating liver tissues of albino rats at 24 and 48hr. after partial hepatectomy. with the following conclusion. (1) EC-2 exerts in vitro an effect to lower the nucleic acid contents in the regenerating liver homogenate. while EC-l does not. (2) EC-2 also lowers the content of glycine in the regenerating tissue homogenate. while EC-l does not. With the above results. possible mechanisms were discussed regarding the mitotic arrest selectively displayed by EC-2.