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Engineering of a multi-functional extracellular matrix protein for immobilization to bone mineral hydroxyapatite

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Authors
Kang, Wonmo; Kim, Tai-Il; Yun, Yerang; Kim, Hae-Won; Jang, Jun-Hyeog
Issue Date
2011-01
Publisher
Springer Verlag
Citation
Biotechnology Letters 33:199-204
Keywords
Bone grafting and repairCell adhesionExtracellular matrixFibronectinHydroxy apatiteOsteocalcin
Abstract
Purpose of Work: We have developed a strategy of designing multi-functional extracellular matrix proteins for functionalizing bone tissue engineering scaffolds and other biomedical surfaces to achieve improvements in bone grafting, bone repair and bone regeneration. We developed a novel extracellular matrix protein designed to have a cell adhesive RGD sequence derived from fibronectin and active functional unit of osteocalcin (OC) containing Ca2+-binding sites for immobilization to mineral component of bone, hydroxyapatite (HA). The fusion protein, designated FNRGD/OC, was expressed in Escherichia coli and purified with affinity chromatography using a His-tag. The resultant FNRGD/OC fusion protein preferentially bound to HA, promoted cell adhesive activity, and stimulated differentiation of MC3T3-E1 cell.
ISSN
0141-5492
Language
English
URI
http://hdl.handle.net/10371/74118
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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