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Engineering of a multi-functional extracellular matrix protein for immobilization to bone mineral hydroxyapatite
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Wonmo | - |
dc.contributor.author | Kim, Tai-Il | - |
dc.contributor.author | Yun, Yerang | - |
dc.contributor.author | Kim, Hae-Won | - |
dc.contributor.author | Jang, Jun-Hyeog | - |
dc.date.accessioned | 2011-10-13T08:16:15Z | - |
dc.date.available | 2011-10-13T08:16:15Z | - |
dc.date.issued | 2011-01 | - |
dc.identifier.citation | Biotechnology Letters 33:199-204 | en |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | https://hdl.handle.net/10371/74118 | - |
dc.description.abstract | Purpose of Work: We have developed a strategy of designing multi-functional extracellular matrix proteins for functionalizing bone tissue engineering scaffolds and other biomedical surfaces to achieve improvements in bone grafting, bone repair and bone regeneration. We developed a novel extracellular matrix protein designed to have a cell adhesive RGD sequence derived from fibronectin and active functional unit of osteocalcin (OC) containing Ca2+-binding sites for immobilization to mineral component of bone, hydroxyapatite (HA). The fusion protein, designated FNRGD/OC, was expressed in Escherichia coli and purified with affinity chromatography using a His-tag. The resultant FNRGD/OC fusion protein preferentially bound to HA, promoted cell adhesive activity, and stimulated differentiation of MC3T3-E1 cell. | en |
dc.description.sponsorship | This work was supported by the Korea
Research Foundation Grant funded by the Korean Government(MOEHRD) (KRF-2007-314-E00183), the Korea Science and Engineering Foundation (KOSEF) Grant funded by the Korea government (MOST) (R01-2007-000-20183), the Grants (#2009-0093829: priority research centers program, and #M10755080002-07N5508-00212) through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology. | en |
dc.language.iso | en | en |
dc.publisher | Springer Verlag | en |
dc.subject | Bone grafting and repair | en |
dc.subject | Cell adhesion | en |
dc.subject | Extracellular matrix | en |
dc.subject | Fibronectin | en |
dc.subject | Hydroxy apatite | en |
dc.subject | Osteocalcin | en |
dc.title | Engineering of a multi-functional extracellular matrix protein for immobilization to bone mineral hydroxyapatite | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 강원모 | - |
dc.contributor.AlternativeAuthor | 김태일 | - |
dc.contributor.AlternativeAuthor | 윤예랑 | - |
dc.contributor.AlternativeAuthor | 김해원 | - |
dc.contributor.AlternativeAuthor | 장준혁 | - |
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