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Electrospun silk fibroin scaffolds with macropores for bone regeneration: An in vitro and in vivo study

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Authors
Park, Sook Young; Ki, Chang Seok; Park, Young Hwan; Jung, Hong Moon; Woo, Kyung Mi; Kim, Hyun Jeong
Issue Date
2010-04
Publisher
Mary Ann Liebert
Citation
Tissue Engineering - Part A 16:4,1271-1279
Abstract
We developed three-dimensional electrospun silk fibroin (ESF) scaffolds with controllable pore size. The purpose of this study was to evaluate ESF scaffolds with pores (P-ESF) for bone regeneration via in vitro and in vivo studies, with a comparison to a commercially available porous three-dimensional polylactic acid (PLA) scaffold. P-ESF supported significantly higher proliferation and alkaline phosphatase activity of osteoblasts than PLA in vitro (p<0.05). Moreover, higher expression levels of activated adhesion-related proteins, including focal adhesion kinase, were observed in the P-ESF than in PLA, as confirmed by western blot analyses. Microcomputed tomography revealed that 78.30% of the original bone volume was attained in the P-ESF implantation group at 7 weeks after critical bone defect formation in rat calvaria. Comparatively, the PLA implantation group showed only 49.31%. Histological evaluation also showed new bone tissue formation upon P-ESF implantation. Taken together, the P-ESF scaffold may be a good bone substitute for bone regeneration.
ISSN
1937-3341
Language
English
URI
http://hdl.handle.net/10371/74174
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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