Publications
Detailed Information
Viral latent membrane protein 1 (LMP-1)–induced CD99 down-regulation in B cells leads to the generation of cells with Hodgkin's and Reed-Sternberg phenotype
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Soon Ha | - |
dc.contributor.author | Shin, Young Kee | - |
dc.contributor.author | Lee, Im-soon | - |
dc.contributor.author | Bae, Young Mee | - |
dc.contributor.author | Sohn, Hae Won | - |
dc.contributor.author | Suh, Young Ho | - |
dc.contributor.author | Ree, Howe J. | - |
dc.contributor.author | Rowe, Martin | - |
dc.contributor.author | Park, Seong Hoe | - |
dc.date.accessioned | 2011-10-18 | - |
dc.date.available | 2011-10-18 | - |
dc.date.issued | 2000 | - |
dc.identifier.citation | Blood; Vol.95, No.1; pp.294-300 | en |
dc.identifier.issn | 0006-4971 | - |
dc.identifier.uri | https://hdl.handle.net/10371/74242 | - |
dc.description.abstract | Recently we reported that the down-regulation of CD99 (Mic2) is a primary requirement for the generation of Hodgkin's and Reed-Sternberg (H-RS) cells seen in Hodgkin's disease. In this study, we provide evidence that the down-regulation of CD99 is induced by high expression of Epstein-Barr virus (EBV) latent membrane protein 1 (LMP-1), which is highly expressed in H-RS cells of EBV-associated Hodgkin's disease. To investigate the effect of LMP-1 on the expression of CD99 in vitro, we established a stable cell line by transfecting an SV40-early promoter driven-LMP-1 expression construct into a neoplastic lymphoblastoid B cell line, IM9, in which the level of endogenous LMP-1 expression is almost negligible. In this cell line, the overexpression of LMP-1 led to the down-regulation of CD99 and the acquisition of morphological and functional characteristics of H-RS cells indistinguishable from those in lymph nodes of Hodgkin's disease patients and in CD99-deficient B cells. In addition, induced LMP-1 expression in an EBV-negative B cell clone, BJAB, directly caused the down-regulation of surface CD99 expression. Northern and Western analysis data, showing that overexpression of LMP-1 negatively influenced the expression of CD99, were supported by experiments in which a CD99 promoter-driven luciferase promoter reporter construct transfected into 293T cells was down-regulated when LMP-1 was coexpressed. Therefore, our data strongly suggest that the EBV LMP-1 protein plays a pivotal role in the down-regulation of CD99 via transcriptional regulation, which leads to the generation of the H-RS cells. | en |
dc.description.sponsorship | Supported by the 99 DiNonA Inc R&D Project, Seoul, Korea. MR was supported by the Leukemia Research Fund, London, UK. | en |
dc.language.iso | en | en |
dc.publisher | American Society of Hematology | en |
dc.title | Viral latent membrane protein 1 (LMP-1)–induced CD99 down-regulation in B cells leads to the generation of cells with Hodgkin's and Reed-Sternberg phenotype | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김순하 | - |
dc.contributor.AlternativeAuthor | 신영기 | - |
dc.contributor.AlternativeAuthor | 이임순 | - |
dc.contributor.AlternativeAuthor | 배영미 | - |
dc.contributor.AlternativeAuthor | 손해원 | - |
dc.contributor.AlternativeAuthor | 서영호 | - |
dc.contributor.AlternativeAuthor | 박성회 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.