Publications

Detailed Information

Viral latent membrane protein 1 (LMP-1)–induced CD99 down-regulation in B cells leads to the generation of cells with Hodgkin's and Reed-Sternberg phenotype

DC Field Value Language
dc.contributor.authorKim, Soon Ha-
dc.contributor.authorShin, Young Kee-
dc.contributor.authorLee, Im-soon-
dc.contributor.authorBae, Young Mee-
dc.contributor.authorSohn, Hae Won-
dc.contributor.authorSuh, Young Ho-
dc.contributor.authorRee, Howe J.-
dc.contributor.authorRowe, Martin-
dc.contributor.authorPark, Seong Hoe-
dc.date.accessioned2011-10-18-
dc.date.available2011-10-18-
dc.date.issued2000-
dc.identifier.citationBlood; Vol.95, No.1; pp.294-300en
dc.identifier.issn0006-4971-
dc.identifier.urihttps://hdl.handle.net/10371/74242-
dc.description.abstractRecently we reported that the down-regulation of CD99 (Mic2) is a primary requirement for the generation of Hodgkin's and Reed-Sternberg (H-RS) cells seen in Hodgkin's disease. In this study, we provide evidence that the down-regulation of CD99 is induced by high expression of Epstein-Barr virus (EBV) latent membrane protein 1 (LMP-1), which is highly expressed in H-RS cells of EBV-associated Hodgkin's disease. To investigate the effect of LMP-1 on the expression of CD99 in vitro, we established a stable cell line by transfecting an SV40-early promoter driven-LMP-1 expression construct into a neoplastic lymphoblastoid B cell line, IM9, in which the level of endogenous LMP-1 expression is almost negligible. In this cell line, the overexpression of LMP-1 led to the down-regulation of CD99 and the acquisition of morphological and functional characteristics of H-RS cells indistinguishable from those in lymph nodes of Hodgkin's disease patients and in CD99-deficient B cells. In addition, induced LMP-1 expression in an EBV-negative B cell clone, BJAB, directly caused the down-regulation of surface CD99 expression. Northern and Western analysis data, showing that overexpression of LMP-1 negatively influenced the expression of CD99, were supported by experiments in which a CD99 promoter-driven luciferase promoter reporter construct transfected into 293T cells was down-regulated when LMP-1 was coexpressed. Therefore, our data strongly suggest that the EBV LMP-1 protein plays a pivotal role in the down-regulation of CD99 via transcriptional regulation, which leads to the generation of the H-RS cells.en
dc.description.sponsorshipSupported by the 99 DiNonA Inc R&D Project, Seoul, Korea. MR was supported by the Leukemia Research Fund, London, UK.en
dc.language.isoenen
dc.publisherAmerican Society of Hematologyen
dc.titleViral latent membrane protein 1 (LMP-1)–induced CD99 down-regulation in B cells leads to the generation of cells with Hodgkin's and Reed-Sternberg phenotypeen
dc.typeArticleen
dc.contributor.AlternativeAuthor김순하-
dc.contributor.AlternativeAuthor신영기-
dc.contributor.AlternativeAuthor이임순-
dc.contributor.AlternativeAuthor배영미-
dc.contributor.AlternativeAuthor손해원-
dc.contributor.AlternativeAuthor서영호-
dc.contributor.AlternativeAuthor박성회-
Appears in Collections:
Files in This Item:
There are no files associated with this item.

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share