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Expression, Solubilization and Purification of a Human Olfactory Receptor from Escherichia coli
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, Hyun Seok | - |
dc.contributor.author | Lee, Sang Hun | - |
dc.contributor.author | Oh, Eun Hae | - |
dc.contributor.author | Park, Tai Hyun | - |
dc.date.accessioned | 2009-08-21T03:23:50Z | - |
dc.date.available | 2009-08-21T03:23:50Z | - |
dc.date.issued | 2009-06-09 | - |
dc.identifier.citation | Curr Microbiol 59:309-314 | en |
dc.identifier.issn | 0343-8651 (print) | - |
dc.identifier.issn | 1432-0991 (online) | - |
dc.identifier.uri | https://hdl.handle.net/10371/7431 | - |
dc.description.abstract | Olfactory receptors pertaining to G protein-coupled receptor (GPCR) are integral membrane proteins composed of seven transmembrane spanning domains. It has been reported that these receptor proteins are difficult to overexpress, solubilize, and purify because of their complicated structures and strong hydrophobicity. In this study, full-length human olfactory receptor (hOR) 2AG1 was overexpressed in E. coli as a fusion protein with a glutathione S-transferase (GST) tag mainly as an inclusion body without any mutations or deletions in the gene. This protein was difficult to solubilize with detergents and chaotropic agents, and only N-lauroyl sarcosine was found to be suitable for solubilizing it. In contrast, Trition X-100 was found to solubilize most of the impurity proteins from the insoluble fraction in E. coli. Based on this observation, we applied a simple and efficient column-free method using these two detergents for the purification of the olfactory receptor protein. In this method, the insoluble fraction of the cell lysate was first treated with Triton X-100 to remove impurity proteins. The remaining insoluble fraction was then further treated with N-lauroyl sarcosine to solubilize the olfactory receptor protein. Milligram quantity of the human olfactory receptor was produced. This is the first report to produce full-length of the olfactory receptor from E. coli. | en |
dc.description.sponsorship | This work was supported by the Korea Science
and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (No. 2009-0080242). This work was also partially supported by the System 2010 program of the Ministry of Knowledge Economy. | en |
dc.language.iso | en | en |
dc.publisher | Springer Verlag | en |
dc.title | Expression, Solubilization and Purification of a Human Olfactory Receptor from Escherichia coli | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 송현석 | - |
dc.contributor.AlternativeAuthor | 이상훈 | - |
dc.contributor.AlternativeAuthor | 오은해 | - |
dc.contributor.AlternativeAuthor | 박태현 | - |
dc.identifier.doi | 10.1007/s00284-009-9435-6 | - |
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