SHERP

Activity-Dependent Neuronal Cell Migration Induced by Electrical Stimulation

Cited 0 time in webofscience Cited 16 time in scopus
Authors
Jeong, Se Hoon; Jun, Sang Beom; Song, Jong Keun; Kim, Sung June
Issue Date
2009-01
Publisher
Springer Verlag
Citation
Med Biol Eng Comput 47:93-99
Keywords
Electrical stimulationNeuronal migrationMicroelectrode arrayAstrocyteNeuronal activity
Abstract
Recently, we found that electrical stimulation
can induce neuronal migration in neural networks cultured
for more than 3 weeks on microelectrode arrays. Immunocytochemistry
data showed that the aggregation of neurons
was related to the emergence of astrocytes in culture. In this
study, when neurons were cocultured with astrocytes, electrical
stimulation could induce the migration of neuronal cell
bodies after only 1 week in culture, while the same stimulation
paradigm caused neural necrosis in neuron-only
cultures. In addition, the stimulation-induced migration was
inhibited by blocking action potentials in neural networks
using the voltage-gated sodium channel blocker, tetrodotoxin.
Immunocytochemistry was performed to monitor
precisely the neuronal migration and count the number of
neurons. These results indicate that neuronal migration of
cell bodies is dependent on neuronal activity evoked by
electrical stimulation and can be enhanced by coculturing with astrocytes. We believe this method can be employed as
a means for modifying neural networks and improving the
interface between electrodes and neurons.
ISSN
0140-0118 (print)
1741-0444 (online)
Language
English
URI
http://hdl.handle.net/10371/7434
DOI
https://doi.org/10.1007/s11517-008-0426-8
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Electrical and Computer Engineering (전기·정보공학부)Journal Papers (저널논문_전기·정보공학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse