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The Leading Blastomere of the 2-Cell Stage Parthenogenetic Porcine Embryo Contributes to the Abembryonic Part First

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dc.contributor.authorPark, Sang Kyu-
dc.contributor.authorWon, Cheolhee-
dc.contributor.authorChoi, Young-Ju-
dc.contributor.authorKang, Hoin-
dc.contributor.authorRoh, Sangho-
dc.date.accessioned2011-10-19T08:36:33Z-
dc.date.available2011-10-19T08:36:33Z-
dc.date.issued2009-
dc.identifier.citationJ. Vet. Med. Sci. 71:569–576en
dc.identifier.issn1347-7439-
dc.identifier.urihttp://hdl.handle.net/10371/74352-
dc.description.abstractPolarity formation in preimplantation embryos is controversial. To investigate the embryonic-abembryonic axis in the pig, porcine parthenotes were used to prevent the topological change caused by polyspermy as well as to avoid the influences of sperm entry position. For lineage tracing, DiI, a fluorescence dye, was injected into only a blastomere of the 2-cell stage embryos. If the first blastomere to divide was labeled, the embryo was included in the leading group, and while all others were included in the lagging group. In 60.5% of the blastocysts in the lagging group, the progeny of the labeled blastomeres formed the inner cell mass (ICM) and adjacent trophectoderm (TE) hemisphere; 62.1% of the blastocysts in the leading group had progeny of the labeled blastomeres distributed only to the TE (opposite of ICM). The rest of the lagging and leading groups showed random distributions. Unlike murine parthenotes, biased mitochondrial distribution was also found in porcine parthenotes (38.1%). Our findings indicate that the `leading' blastomere of the 2-cell porcine parthenote forms the distal TE (abembryonic) and that the `lagging' blastomere forms the remaining portion of the blastocyst, including the ICM (embryonic). Biased distribution of mitochondria in each 2-cell blastomere may contribute partly to this event.en
dc.description.sponsorshipThis study was supported by Korea Science and Engineering Foundation(KOSEF) grants funded by the Government of the Republic of Korea(MOST;M10641000001-06N4100-00110 and R01-2007-000-10316-0).en
dc.language.isoenen
dc.publisherJapanese Society of Veterinary Scienceen
dc.subjectembryonic-abembryonic polarityen
dc.subjectmitochondriaen
dc.subjectparthenogenesisen
dc.subjectporcineen
dc.titleThe Leading Blastomere of the 2-Cell Stage Parthenogenetic Porcine Embryo Contributes to the Abembryonic Part Firsten
dc.typeArticleen
dc.contributor.AlternativeAuthor박상규-
dc.contributor.AlternativeAuthor원철희-
dc.contributor.AlternativeAuthor최영주-
dc.contributor.AlternativeAuthor강호인-
dc.contributor.AlternativeAuthor노상호-
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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