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Porcine nuclear transfer using somatic donor cells altered to express male germ cell function

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Authors
Roh, Sangho; Choi, Hye-Yeon; Park, Sang Kyu; Won, Cheolhee; Kim, Bong-Woo; Kim, Jung-Hyun; Kang, Hoin; Lee, Eung-Ryoung; Cho, Ssang-Goo
Issue Date
2009-07
Publisher
CSIRO Publishing
Citation
Mol. Reprod. Dev 21,882–891
Keywords
reprogrammingtestis extracts
Abstract
Recent studies reported that the direct transformation of one differentiated somatic cell type into another is possible. In the present study, we were able to modulate the cell fate of somatic cells to take on male germ cell function by introducing cell extracts derived from porcine testis tissue. Fibroblasts were treated with streptolysin O, which reversibly permeabilises the plasma membrane, and incubated with testis extracts. Our results showed that the testis extracts (TE) could activate expression of male germ cell-specific genes, implying that TE can provide regulatory components required for altering the cell fate of fibroblasts. Male germ cell function was sustained for more than 10 days after the introduction of TE. In addition, a single TE-treated cell was injected directly into the cytoplasm of in vitro-matured porcine oocytes. The rate of blastocyst formation was significantly higher in the TE-treated nuclear donor cell group than in the control cell group. The expression level of Nanog, Sox9 and Eomes was drastically increased when altered cells were used as donor nuclei. Our results suggest that TE can be used to alter the cell fate of fibroblasts to express male germ cell function and improve the developmental efficiency of the nuclear transfer porcine embryos.
ISSN
1448-5990
Language
English
URI
http://hdl.handle.net/10371/74354
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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