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Expression of Amino Acid Transporter LAT1 During Ameloblast Differentiation

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Authors
Kim, Sang-Bong; Kim, Do-Kyung; Kim, Chun-Sung; Kook, Joong-Ki; Park, Joo-Cheol; Kim, Heung-Joong
Issue Date
2009-09
Publisher
Korean Acadamy of Oral Biology
Citation
Int. J Oral Biol. 2009;34:143-150
Keywords
amino acid transporterLAT1ameloblastdifferentiation
Abstract
Amino acid transporters play important roles in supplying nutrients to cells. In our current study, we investigated the expression of LAT1 and measured the amino acid uptake in ameloblast cultures to further elucidate the roles of this transporter during the differentiation of these cells. RT-PCR, observations of cell morphology, Alizaline red-S staining, and uptake analyses were performed following the experimental induction of differentiation in the cultures. LAT1 mRNA was detectable and found to gradually increase over time whereas LAT2 mRNA was not evident in the ameloblast cultures. Transcripts of 4F2hc, a cofactor of LAT1 and LAT2, were also found to be expressed in ameloblast cultures and increase with time. Amelogenin mRNA was expressed in the early stage ameloblast cultures. L-leucine uptake was observed to increase over 14 days of growth in culture. Our data suggest that LAT1 has a key role in the differentiation of ameloblasts and in providing these cells with neutral amino acids, including several essential amino acids.
ISSN
1226-7155
Language
Korean
URI
http://hdl.handle.net/10371/74381
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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