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Expression of Amino Acid Transporter LAT1 During Ameloblast Differentiation

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dc.contributor.authorKim, Sang-Bong-
dc.contributor.authorKim, Do-Kyung-
dc.contributor.authorKim, Chun-Sung-
dc.contributor.authorKook, Joong-Ki-
dc.contributor.authorPark, Joo-Cheol-
dc.contributor.authorKim, Heung-Joong-
dc.date.accessioned2011-10-20T05:06:28Z-
dc.date.available2011-10-20T05:06:28Z-
dc.date.issued2009-09-
dc.identifier.citationInt. J Oral Biol. 2009;34:143-150en
dc.identifier.issn1226-7155-
dc.identifier.urihttp://hdl.handle.net/10371/74381-
dc.description.abstractAmino acid transporters play important roles in supplying nutrients to cells. In our current study, we investigated the expression of LAT1 and measured the amino acid uptake in ameloblast cultures to further elucidate the roles of this transporter during the differentiation of these cells. RT-PCR, observations of cell morphology, Alizaline red-S staining, and uptake analyses were performed following the experimental induction of differentiation in the cultures. LAT1 mRNA was detectable and found to gradually increase over time whereas LAT2 mRNA was not evident in the ameloblast cultures. Transcripts of 4F2hc, a cofactor of LAT1 and LAT2, were also found to be expressed in ameloblast cultures and increase with time. Amelogenin mRNA was expressed in the early stage ameloblast cultures. L-leucine uptake was observed to increase over 14 days of growth in culture. Our data suggest that LAT1 has a key role in the differentiation of ameloblasts and in providing these cells with neutral amino acids, including several essential amino acids.en
dc.description.sponsorship이 연구는 2008년도 조선대학교 교내연구비 지원에 의해 수행되었음.en
dc.language.isokoen
dc.publisherKorean Acadamy of Oral Biologyen
dc.subjectamino acid transporteren
dc.subjectLAT1en
dc.subjectameloblasten
dc.subjectdifferentiationen
dc.titleExpression of Amino Acid Transporter LAT1 During Ameloblast Differentiationen
dc.typeArticleen
dc.contributor.AlternativeAuthor김상봉-
dc.contributor.AlternativeAuthor김도경-
dc.contributor.AlternativeAuthor김준성-
dc.contributor.AlternativeAuthor국중기-
dc.contributor.AlternativeAuthor박주철-
dc.contributor.AlternativeAuthor김흥중-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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