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Immune stimulating activity of an atrophic rhinitis vaccine associated to pegylated chitosan microspheres in vitro

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Authors
Jiang, Hu-Lin; Park, In-Kyu; Kang, Mi-Lan; Yoo, Han Sang; Choi, Yun-Jaie; Akaike, Toshihiro; Cho, Chong-Su
Issue Date
2007-02-02
Publisher
Wiley-Blackwell
Citation
Polym. Adv. Technol. 2007; 18: 220-225
Keywords
chitosanBordetella bronchiseptica dermonecrotoxindrug delivery systemstumor necrosis factor
Abstract
It has previously been found that chitosan microspheres are easily aggregated due to their physical and storage instabilities. In this study, to overcome their instability, chitosan was covalently conjugated with poly(ethylene glycol). Pegylated chitosan microspheres were prepared through the ionic gelation process of pegylated chitosan with tripolyphosphate. Bordetella bronchiseptica dermonecrotoxin, major virulence factor of atrophic rhinitis causative agent, was loaded onto pegylated chitosan microspheres for nasal vaccination. Average particle sizes of Bordetella bronchiseptica dermonecrotoxin-loaded pegylated chitosan microspheres were 5.47 µm. Microspheres obtained from pegylated chitosan microspheres were physically more stable than those from chitosan microspheres, and Bordetella bronchiseptica dermonecrotoxin-loaded pegylated chitosan microspheres released more Bordetella bronchiseptica dermonecrotoxin than Bordetella bronchiseptica dermonecrotoxin-loaded chitosan microspheres in vitro. Macrophage RAW264.7 cells stimulated with Bordetella bronchiseptica dermonecrotoxin-loaded pegylated chitosan microspheres gradually secreted tumor necrosis factor and nitric oxide, suggesting that pegylated chitosan microspheres are very promising vaccine delivery systems.
ISSN
1042-7147
Language
English
URI
http://hdl.handle.net/10371/7471
DOI
https://doi.org/10.1002/pat.861
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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