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Soft lithographic patterning of supported lipid bilayers onto a surface and inside microfluidic channels

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Authors
Kim, Pilnam; Lee, Sang Eun; Jung, Ho Sup; Lee, Hea Yeon; Kawai, Tomoji; Suh, Kahp Y.
Issue Date
2006
Publisher
Royal Society of Chemistry
Citation
Lab Chip, 2006, 6, 54-59
Keywords
SELF-ASSEMBLED MONOLAYERSPHOSPHOLIPID-BILAYERSCOMB POLYMERMEMBRANESARRAYSMICROSTRUCTURESPOLY(DIMETHYLSILOXANE)
Abstract
We present simple soft lithographic methods for patterning supported lipid bilayer (SLB) membranes onto a surface and inside microfluidic channels. Micropatterns of polyethylene glycol (PEG)-based polymers were fabricated on glass substrates by microcontact printing or capillary moulding. The patterned PEG surfaces have shown 97 +/- 0.5% reduction in lipid adsorption onto two dimensional surfaces and 95 +/- 1.2% reduction inside microfluidic channels in comparison to glass control. Atomic force microscopy measurements indicated that the deposition of lipid vesicles led to the formation of SLB membranes by vesicle fusion due to hydrophilic interactions with the exposed substrate. Furthermore, the functionality of the patterned SLBs was tested by measuring the binding interactions between biotin (ligand)-labeled lipid bilayer and streptavidin (receptor). SLB arrays were fabricated with spatial resolution down to similar to 500 nm on flat substrate and similar to 1 mu m inside microfluidic channels, respectively.
ISSN
1473-0197
Language
English
URI
http://hdl.handle.net/10371/7506
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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