Browse

In vitro reconstitution of the interactions in the PIDDosome

Cited 17 time in Web of Science Cited 17 time in Scopus
Authors
Jang, Tae-ho; Zheng, Chao; Wu, Hao; Park, Hyun Ho; Jeon, Ju-Hong
Issue Date
2010-12
Publisher
SPRINGER
Citation
APOPTOSIS; Vol.15 12; 1444-1452
Keywords
ApoptosisCaspase-2RAIDDPIDDosomePIDDInflammation
Abstract
Caspase-2 is critical for genotoxic stress induced apoptosis and is activated by formation of the PIDDosome, an oligomeric caspase-2 activating complex. The PIDDosome comprises three protein components, PIDD, RAIDD, and caspase-2. RAIDD contains both a death domain (DD) and a caspase recruitment domain (CARD). It acts as the bridge to recruit PIDD using the DD: DD interaction and to recruit caspase-2 via the CARD: CARD interaction. Here we report biochemical characterization and in vitro reconstitution of the core interactions in the PIDDosome. We show that RAIDD CARD and RAIDD DD interact with their binding partners, caspase-2 CARD and PIDD DD, respectively. However, full-length RAIDD fails to interact with either caspase-2 CARD or PIDD DD under a physiological buffer condition. We reveal that this lack of interaction of full-length RAIDD is not due to its tendency to aggregate under the physiological buffer condition, as decreasing full-length RAIDD aggregation using high salt or high pH is not able to promote complex formation. Instead, full-length RAIDD forms both binary and ternary complexes under a low salt condition. Successful in vitro reconstitution of the ternary complex provides a basis for further structural studies of the PIDDosome.
ISSN
1360-8185
Language
English
URI
http://hdl.handle.net/10371/76208
DOI
https://doi.org/10.1007/s10495-010-0544-2
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse