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In vitro reconstitution of the interactions in the PIDDosome
DC Field | Value | Language |
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dc.contributor.author | Jang, Tae-ho | - |
dc.contributor.author | Zheng, Chao | - |
dc.contributor.author | Wu, Hao | - |
dc.contributor.author | Park, Hyun Ho | - |
dc.contributor.author | Jeon, Ju-Hong | - |
dc.date.accessioned | 2012-05-22T02:24:15Z | - |
dc.date.available | 2012-05-22T02:24:15Z | - |
dc.date.issued | 2010-12 | - |
dc.identifier.citation | APOPTOSIS; Vol.15 12; 1444-1452 | ko_KR |
dc.identifier.issn | 1360-8185 | - |
dc.identifier.uri | https://hdl.handle.net/10371/76208 | - |
dc.description.abstract | Caspase-2 is critical for genotoxic stress induced apoptosis and is activated by formation of the PIDDosome, an oligomeric caspase-2 activating complex. The PIDDosome comprises three protein components, PIDD, RAIDD, and caspase-2. RAIDD contains both a death domain (DD) and a caspase recruitment domain (CARD). It acts as the bridge to recruit PIDD using the DD: DD interaction and to recruit caspase-2 via the CARD: CARD interaction. Here we report biochemical characterization and in vitro reconstitution of the core interactions in the PIDDosome. We show that RAIDD CARD and RAIDD DD interact with their binding partners, caspase-2 CARD and PIDD DD, respectively. However, full-length RAIDD fails to interact with either caspase-2 CARD or PIDD DD under a physiological buffer condition. We reveal that this lack of interaction of full-length RAIDD is not due to its tendency to aggregate under the physiological buffer condition, as decreasing full-length RAIDD aggregation using high salt or high pH is not able to promote complex formation. Instead, full-length RAIDD forms both binary and ternary complexes under a low salt condition. Successful in vitro reconstitution of the ternary complex provides a basis for further structural studies of the PIDDosome. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | SPRINGER | ko_KR |
dc.subject | Apoptosis | ko_KR |
dc.subject | Caspase-2 | ko_KR |
dc.subject | RAIDD | ko_KR |
dc.subject | PIDDosome | ko_KR |
dc.subject | PIDD | ko_KR |
dc.subject | Inflammation | ko_KR |
dc.title | In vitro reconstitution of the interactions in the PIDDosome | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 장태호 | - |
dc.contributor.AlternativeAuthor | 박현호 | - |
dc.contributor.AlternativeAuthor | 전주홍 | - |
dc.identifier.doi | 10.1007/s10495-010-0544-2 | - |
dc.citation.journaltitle | APOPTOSIS | - |
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