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Electrospray ionization-tandem mass spectrometry analysis of the mycolic acid profiles for the identification of common clinical isolates of mycobacterial species
DC Field | Value | Language |
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dc.contributor.author | Song, Sang Hoon | - |
dc.contributor.author | Park, Kyoung Un | - |
dc.contributor.author | Lee, Jae Ho | - |
dc.contributor.author | Kim, Eui Chong | - |
dc.contributor.author | Song, Junghan | - |
dc.contributor.author | Kim, Jin Q. | - |
dc.date.accessioned | 2012-05-22T07:11:14Z | - |
dc.date.available | 2012-05-22T07:11:14Z | - |
dc.date.issued | 2009-05 | - |
dc.identifier.citation | JOURNAL OF MICROBIOLOGICAL METHODS; Vol.77 2; 165-177 | ko_KR |
dc.identifier.issn | 0167-7012 | - |
dc.identifier.uri | https://hdl.handle.net/10371/76247 | - |
dc.description.abstract | Mycolic acids are unique and complex molecular structures found in mycobacterial species. In the present study, we investigated whether electrospray ionization-tandem mass spectrometry (ESI-MS/MS) can be used to identify mycobacterial species based on their mycolic acid profiles. Clinical isolates of Mycobacterium tuberculosis complex and 18 nontuberculosis mycobacterial (NTM) species identified by PCR-restriction fragment length polymorphism (RFLP) or real-time PCR were used for this analysis. Crude lipid extracts were prepared by saponifying 1-2 colonies of individual isolates of mycobacterial species and by chloroform and methanol (2:1, v/v) extraction. ESI-MS/MS in negative ion mode with high cone voltage and collision energy was used for mycolic acid profiling analysis. Combinatorial precursor ion scans of m/z 395, 367, and 339 in the range of m/z 1000-1400 resulted in spectra specific to individual mycobacteria. M. tuberculosis complex and M. pulveris showed major ions by performing precursor ion scans on m/z 395 and 367, while other NTM species showed major ions by performing scans on m/z 367 and 339. The different NTM species examined showed different species dependent mycolic acid profiles. In conclusion, we describe a rapid, reliable, and informative ESI-MS/MS protocol for mycolic acid profiling in mycobacterial species, which allows mycobacterial species to be easily identified in clinical laboratories. (C) 2009 Elsevier B.V. All rights reserved. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | ELSEVIER SCIENCE BV | ko_KR |
dc.subject | Mycolic acid | ko_KR |
dc.subject | Mass spectrometry | ko_KR |
dc.subject | Electrospray ionization | ko_KR |
dc.subject | Tuberculosis | ko_KR |
dc.subject | Nontuberculosis mycobacteria | ko_KR |
dc.title | Electrospray ionization-tandem mass spectrometry analysis of the mycolic acid profiles for the identification of common clinical isolates of mycobacterial species | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 송상훈 | - |
dc.contributor.AlternativeAuthor | 박경운 | - |
dc.contributor.AlternativeAuthor | 이재호 | - |
dc.contributor.AlternativeAuthor | 김의종 | - |
dc.contributor.AlternativeAuthor | 김진규. | - |
dc.contributor.AlternativeAuthor | 송정한 | - |
dc.identifier.doi | 10.1016/j.mimet.2009.01.023 | - |
dc.citation.journaltitle | JOURNAL OF MICROBIOLOGICAL METHODS | - |
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dc.description.tc | 5 | - |
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