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Hypoxia-inducible factor 1 alpha is deregulated by the serum of rats with adjuvant-induced arthritis

Cited 6 time in Web of Science Cited 6 time in Scopus
Authors
Kim, Hye-Lim; Cho, Young-Suk; Choi, Hong; Chun, Yang-Sook; Park, Jong-Wan; Lee, Zang Hee
Issue Date
2009-01-02
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS; Vol.378 1; 123-128
Keywords
Rheumatoid arthritisARTInsulinHypoxia-inducible factor-1SerumHypoxic diseases
Abstract
Rheumatoid arthritis (RA) is known to be associated with increased risks of hypoxia-related diseases, whose progresses are critically determined by HIF-1 alpha, The authors hypothesized that the hypoxia-related complications of RA are associated with HIF-1 alpha deregulation by some factor(s) in RA serum. Arthritis was induced in female Lewis rats by injecting complete Freund`s adjuvant. The effects of arthritic rat serum (ARS) on hypoxic responses were investigated by incubating Hep3B cells in ARS. In the presence of ARS, HIF-1 alpha was down-regulated and inactivated under hypoxic conditions. ARS inactivated AKT and mTOR, which led to impaired HIF-1 alpha protein synthesis. Furthermore, insulin was found to be deficient in ARS and insulin supplementation fully recovered HIF-1 alpha synthesis with AKT and mTOR activation. These results suggest that HIF-1 alpha deregulation by components in serum is responsible for the RA-associated aggravation of hypoxic diseases in extra-articular tissues. (C) 2008 Elsevier Inc. All rights reserved.
ISSN
0006-291X
Language
English
URI
http://hdl.handle.net/10371/76248
DOI
https://doi.org/10.1016/j.bbrc.2008.11.013
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
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