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Wnt inhibitory factor (WIF)-1 inhibits osteoblastic differentiation in mouse embryonic mesenchymal cells

Cited 35 time in Web of Science Cited 35 time in Scopus
Authors
Cho, Sun Wook; Yang, Jae-Yeon; Sun, Hyun Jin; Jung, Ju Yeon; Cho, Hwa Young; Kim, Sang Wan; Shin, Chan Soo; Kim, Seong Yeon; Choi, Hyung Jin; Her, Sun Ju
Issue Date
2009-06
Publisher
ELSEVIER SCIENCE INC
Citation
BONE; Vol.44 6; 1069-1077
Keywords
Wnt inhibitory factor-1AdipogenesisMesenchymal cellOsteoblastic differentiationWnt
Abstract
Wnt inhibitory factor (WIF)-1 belongs to the members of secreted modulators of Wnt proteins. Secreted frizzled-related proteins (sFRPs), another member of Wnt modulators, have been shown to play differential roles in Writ signaling depending on the subtypes and cell models. This Study was undertaken to investigate the functional role of WIF-1 in osteoblastic differentiation of mouse mesenchymal C3H10T1/2 cells. C3H10T1/2 cells express endogenous WIF-1 and its expression level decreases during osteoblastogenesis. Treatment of C3H10T1/2 cells with WIF-1 significantly reduced alkaline phosphatase (ALP) activities induced by either osteogenic medium (OM, ascorbic acid and beta-glycerophosphate) or Wnt-3a conditioned medium (CM) in a dose-dependent manner. In contrast, the expression level of endogenous WIF-1 increased during adipogenesis and WIF-1 treatment resulted in increased adipogenesis. C3H10T1/2 cells transduced with WIF-1 retrovirus also exhibited reduced ALP activity and decreased mRNA expression of Runx2, collagen type 1, ALP and cisteocalcin during osteoblastic differentiation compared to empty virus-transduced cells. Moreover, treatment with WIF-1 dose-dependently attenuates beta-catenin/T-cell factor (TCF) transcriptional activity in this cell line. Finally, knockdown of WIF-1 in C3H10T1/2 cells by RNA interference leads to increase in ALP activities. Collectively, these results indicate that WIF-1 plays as a negative regulator of osteoblastic differentiation in mouse mesenchymal C3H10T1/2 cells in vitro. (C) 2009 Elsevier Inc. All rights reserved.
ISSN
8756-3282
Language
English
URI
http://hdl.handle.net/10371/76446
DOI
https://doi.org/10.1016/j.bone.2009.02.012
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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