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Novel Embryoid Body-Based Method to Derive Mesenchymal Stem Cells from Human Embryonic Stem Cells

Cited 46 time in Web of Science Cited 49 time in Scopus
Authors
Lee, Eun Ju; Lee, Ha-Neul; Kang, Hyun-Jae; Kim, Keum-Hyun; Cho, Hyun-Jai; Chung, Hyung-Min; Cho, Mee-Young; Moon, Shin-Yong; Kim, Hyo-Soo; Park, Young-Bae; Oh, Sun-Kyung; Cho, Jaejin; Lee, Jaewon; Hur, Jin
Issue Date
2010-02
Publisher
MARY ANN LIEBERT INC
Citation
TISSUE ENGINEERING PART A; Vol.16 2; 705-715
Abstract
Application of human embryonic stem cells (hESCs) to stem-cell therapy is not feasible because of the risk of tumorigenicity and rejection. In contrast, human mesenchymal stem cells (hMSCs) are free from the risk of tumorigenicity and also have immune privilege. However, hMSCs obtained from adults have infinite variety in terms of the biological characteristics and functionality. We report here a new derivation method of hMSCs from hESCs. The derivation of hMSCs from three different hESC lines (SNUhES3, CHA3-hESC, and H9) was performed by embryoid bodies formation and subsequent culture with stage-different media without using inductive xenogenic feeder and mechanical selection procedure. The derived cells were morphologically similar to the unique fingerprint-like pattern of hMSCs and grew stably for at least 35 passages in vitro. These cells had hMSCs-like immunophenotypes: negative for CD34 and CD45; positive for CD29, CD44, CD73, CD90, and CD105. They could be differentiated into multiple lineages including osteocytes, chondrocytes, adipocytes, and myocytes. They maintained normal karyotype during the long-term cultivation and did not show tumorigenicity when transplanted into the immunodeficient mice. In conclusion, the new embryoid body-based derivation method of hMSCs from hESCs is simple, safe, and reproducible in three different hESC lines. We expect that this method will provide a more effective and powerful tool to derive hMSCs from various hESC lines.
ISSN
1937-3341
Language
English
URI
http://hdl.handle.net/10371/76546
DOI
https://doi.org/10.1089/ten.tea.2008.0596
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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