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Identification of Novel Methylation Markers in Hepatocellular Carcinoma using a Methylation Array

Cited 39 time in Web of Science Cited 40 time in Scopus
Authors
Shin, So Hyun; Kim, Baek-hui; Jang, Ja-June; Suh, Kyung Suk; Kang, Gyeong Hoon
Issue Date
2010-08
Publisher
KOREAN ACAD MEDICAL SCIENCES
Citation
JOURNAL OF KOREAN MEDICAL SCIENCE; Vol.25 8; 1152-1159
Keywords
Bead ArrayCarcinomaCpG IslandsDNA MethylationHepatocellular
Abstract
Promoter CpG island hypermethylation has become recognized as an important mechanism for inactivating tumor suppressor genes or tumor-related genes in human cancers of various tissues. Gene inactivation in association with promoter CpG island hypermethylation has been reported to be four times more frequent than genetic changes in human colorectal cancers. Hepatocellular carcinoma is also one of the human cancer types in which aberrant promoter CpG island hypermethylation is frequently found. However, the number of genes identified to date as hypermethylated for hepatocellular carcinoma (HCC) is fewer than that for colorectal cancer or gastric cancer, which can be attributed to fewer attempts to perform genome-wide methylation profiling for HCC. In the present study, we used bead-array technology and coupled methylation-specific PCR to identify new genes showing cancer-specific methylation in HCC. Twenty-four new genes have been identified as hypermethylated at their promoter CpG island loci in a cancer-specific manner. Of these, TNFRSF10C, HOXA9, NPY, and IRF5 were frequently hypermethylated in hepatocellular carcinoma tissue samples and their methylation was found to be closely associated with inactivation of gene expression. Further study will be required to elucidate the clinicopathological implications of these newly found DNA methylation markers in hepatocellular carcinoma.
ISSN
1011-8934
Language
English
URI
http://hdl.handle.net/10371/76670
DOI
https://doi.org/10.3346/jkms.2010.25.8.1152
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College of Medicine/School of Medicine (의과대학/대학원)Pathology (병리학전공)Journal Papers (저널논문_병리학전공)
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