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Reliability of chromogenic in situ hybridization for epidermal growth factor receptor gene copy number detection in non-small-cell lung carcinomas: A comparison with fluorescence in situ hybridization study

Cited 24 time in Web of Science Cited 21 time in Scopus
Authors
Yoo, Seol Bong; Lee, Hyun Ju; Park, Jung Ok; Choe, Gheeyoung; Seo, Jeong-Wook; Chung, Jin-Haeng; Chung, Doo Hyun
Issue Date
2010-03
Publisher
ELSEVIER IRELAND LTD
Citation
LUNG CANCER; Vol.67 3; 301-305
Keywords
EGFRReproducibilityNon-small-cell carcinomaFluorescence in situ hybridizationChromogenic in situ hybridization
Abstract
Fluorescence in situ hybridization (FISH) has been known to be the most representative and standardized test for assessing gene amplification However. FISH requires a fluorescence microscope, the signals are labile and rapidly fade over time. Recently, chromogenic in situ hybridization (CISH) has emerged as a potential alternative to FISH The aim of this study is to test the reliability of CISH technique for the detection of epidermal growth factor receptor (EGFR) gene amplification in non-small-cell lung carcinomas (NSCLC), to compare CISH results with FISH. A total of 277 formalin-fixed and paraffin embedded NSCLC tissue samples were retrieved nom the surgical pathology archives at Seoul National University Bundang Hospital CISH and FISH examinations were performed to test EGFR gene amplification status There was high concordance in the assessment of EGFR gene copy number between CISH and FISH tests (Kappa coefficient = 0.83) Excellent concordance was shown between two observers on the interpretation of the CISH results (Kappa coefficient = 0 90) In conclusion, CISH result is highly reproducible, accurate and practical method to determine EGFR gene amplification in NSCLC In addition, CISH allows a concurrent analysis of histological features of the tumors and gene copy numbers. (C) 2009 Elsevier Ireland Ltd All rights reserved.
ISSN
0169-5002
Language
English
URI
http://hdl.handle.net/10371/76702
DOI
https://doi.org/10.1016/j.lungcan.2009.05.002
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College of Medicine/School of Medicine (의과대학/대학원)Pathology (병리학전공)Journal Papers (저널논문_병리학전공)
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