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Rapid and high-throughput analysis of N-glycans from ovarian cancer serum using a 96-well plate platform

Cited 25 time in Web of Science Cited 26 time in Scopus
Authors
Kim, Yun-Gon; Jeong, Hee-Jin; Jang, Kyoung-Soon; Yang, Yung-Hun; Chung, Junho; Kim, Byung-Gee; Song, Yong-Sang
Issue Date
2009-08-15
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
ANALYTICAL BIOCHEMISTRY; Vol.391 2; 151-153
Abstract
We present a rapid and high-throughput human serum N-glycan preparation technology using 96-well plate-based procedures, The released N-glycans front polyvinylidene fluoride (PVDF) membrane filter plate are subsequently loaded to porous graphitic carbon (PGC) containing a 96-well plate to remove salts and other contaminants without sacrificing accuracy or reproducibility. This robust glycan Preparation technology is applied to ovarian cancer diagnosis using 5 mu l of patient Serum. (C) 2009 Elsevier Inc. All rights reserved.
ISSN
0003-2697
Language
English
URI
http://hdl.handle.net/10371/76753
DOI
https://doi.org/10.1016/j.ab.2009.05.015
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College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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