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Involvement of Both Extrinsic and Intrinsic Apoptotic Pathways in Apoptosis Induced by Genistein in Human Cervical Cancer Cells

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dc.contributor.authorKim, Su-Hyeon-
dc.contributor.authorKim, Su-Hyeong-
dc.contributor.authorLee, Sang-Chul-
dc.contributor.authorSong, Yong-Sang-
dc.date.accessioned2012-06-04T02:01:59Z-
dc.date.available2012-06-04T02:01:59Z-
dc.date.issued2009-
dc.identifier.citationNATURAL COMPOUNDS AND THEIR ROLE IN APOPTOTIC CELL SIGNALING PATHWAYS: Ann. N.Y. Acad. Sci.; Vol.1171 ; 196-201ko_KR
dc.identifier.issn0077-8923-
dc.identifier.urihttp://hdl.handle.net/10371/76754-
dc.description.abstractGenistein, a naturally occurring isoflavonoid abundant in soy products, has anticancer activity in multiple tumor cells. In this study, we evaluated the apoptotic effect of genistein on cervical cancer cells and its mechanism of apoptosis. Genistein inhibited the proliferation of cervical cancer cells (HeLa, CaSki, and C33A). HeLa cells were the most sensitive to genistein, whereas CaSki and C33A cells were less sensitive. Sub-G, analysis showed that genistein increased apoptotic cells up to 45% at a concentration of 60 mu mol/L in HeLa cells, whereas it produced 21% and 17% apoptotic cells in CaSki and C33A cells, respectively, at the same concentration. To determine the apoptotic pathway induced by genistein in the cervical cancer cells, we assessed activation of caspase-3, -8, and -9 by immunoblotting. Procaspase-3, -8, and -9 were decreased and PARP cleavage increased in a time-dependent manner after the treatment of genistein in HeLa cells. Also, inhibition of caspase-3, -8, and -9 with pharmacological inhibitors reduced genistein-mediated apoptosis. Interestingly, inhibition of caspase-8 resulted in remarkable reduction of genistein-induced apoptosis. Bax expression was increased and total bid decreased, whereas bcl-2 level was not changed by genistein. Taken together, these results suggest that genistein could induce apoptosis through both extrinsic and intrinsic pathways in human cervical cancer cells.ko_KR
dc.language.isoenko_KR
dc.publisherBLACKWELL PUBLISHINGko_KR
dc.subjectgenisteinko_KR
dc.subjectapoptosisko_KR
dc.subjectcervical cancerko_KR
dc.titleInvolvement of Both Extrinsic and Intrinsic Apoptotic Pathways in Apoptosis Induced by Genistein in Human Cervical Cancer Cellsko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor김수현-
dc.contributor.AlternativeAuthor김수형-
dc.contributor.AlternativeAuthor이상철-
dc.contributor.AlternativeAuthor송용상-
dc.identifier.doi10.1111/j.1749-6632.2009.04902.x-
dc.citation.journaltitleNATURAL COMPOUNDS AND THEIR ROLE IN APOPTOTIC CELL SIGNALING PATHWAYS-
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College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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