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Development and Application of Multiprobe Real-Time PCR Method Targeting the hsp65 Gene for Differentiation of Mycobacterium Species from Isolates and Sputum Specimens
DC Field | Value | Language |
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dc.contributor.author | Kim, Kijeong | - |
dc.contributor.author | Lee, Hyungki | - |
dc.contributor.author | Lee, Mi-Kyung | - |
dc.contributor.author | Lee, Seoung-Ae | - |
dc.contributor.author | Lim, Seong Yong | - |
dc.contributor.author | Yim, Jae-Joon | - |
dc.contributor.author | Kim, Wonyong | - |
dc.contributor.author | Kook, Yoon-Hoh | - |
dc.contributor.author | Kim, Bum-Joon | - |
dc.contributor.author | Chung, Sang-In | - |
dc.contributor.author | Munkhtsetseg, Bazarragchaa | - |
dc.contributor.author | Koh, Won-Jung | - |
dc.contributor.author | Shim, Tae-Sun | - |
dc.date.accessioned | 2012-06-26T05:54:13Z | - |
dc.date.available | 2012-06-26T05:54:13Z | - |
dc.date.issued | 2010-09 | - |
dc.identifier.citation | JOURNAL OF CLINICAL MICROBIOLOGY; Vol.48 9; 3073-3080 | ko_KR |
dc.identifier.issn | 0095-1137 | - |
dc.identifier.uri | https://hdl.handle.net/10371/77443 | - |
dc.description.abstract | We developed a multiprobe real-time PCR assay targeting hsp65 (HMPRT-PCR) to detect and identify mycobacterial isolates and isolates directly from sputum specimens. Primers and probes for HMPRT-PCR were designed on the basis of the hsp65 gene sequence, enabling the recognition of seven pathogenic mycobacteria, including Mycobacterium tuberculosis, M. avium, M. intracellulare, M. kansasii, M. abscessus, M. massiliense, and M. fortuitum. This technique was applied to 24 reference and 133 clinical isolates and differentiated between all strains with 100% sensitivity and specificity. Furthermore, this method was applied to sputum specimens from 117 consecutive smear-positive patients with smear results of from a trace to 3+. These results were then compared to those obtained using the rpoB PCR-restriction analysis method with samples from cultures of the same sputum specimens. The HMPRT-PCR method correctly identified the mycobacteria in 89 samples (76.0%, 89/117), and moreover, the sensitivity level was increased to 94.3% (50/53) for sputa with an acid-fast bacillus score equal to or greater than 2+. Our data suggest that this novel HMPRT-PCR method could be a promising approach for detecting pathogenic mycobacterial species from sputum samples and culture isolates routinely in a clinical setting. | ko_KR |
dc.description.sponsorship | This study was supported by grant A101205 from the Korean
Healthcare Technology R&D project, Ministry for Health, Welfare & Family Affairs, Republic of Korea, and in part supported by grant 04-2008-0860 from the SNUH Research Fund. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | AMER SOC MICROBIOLOGY | ko_KR |
dc.title | Development and Application of Multiprobe Real-Time PCR Method Targeting the hsp65 Gene for Differentiation of Mycobacterium Species from Isolates and Sputum Specimens | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김기정 | - |
dc.contributor.AlternativeAuthor | 이형기 | - |
dc.contributor.AlternativeAuthor | 이미경 | - |
dc.contributor.AlternativeAuthor | 이승애 | - |
dc.contributor.AlternativeAuthor | 심태선 | - |
dc.contributor.AlternativeAuthor | 임성용 | - |
dc.contributor.AlternativeAuthor | 고원정 | - |
dc.contributor.AlternativeAuthor | 임재준 | - |
dc.contributor.AlternativeAuthor | 김원용 | - |
dc.contributor.AlternativeAuthor | 정상인 | - |
dc.contributor.AlternativeAuthor | 국윤호 | - |
dc.contributor.AlternativeAuthor | 김범준 | - |
dc.identifier.doi | 10.1128/JCM.00939-10 | - |
dc.citation.journaltitle | JOURNAL OF CLINICAL MICROBIOLOGY | - |
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