Browse

An Active Part of Artemisia sacrorum Ledeb. Attenuates Hepatic Lipid Accumulation through Activating AMP-Activated Protein Kinase in Human HepG2 Cells

Cited 15 time in Web of Science Cited 15 time in Scopus
Authors
Yuan, Hai-Dan; Yuan, Hai-Ying; Chung, Sung-Hyun; Jin, Guang-Zhu; Piao, Guang-Chun
Issue Date
2010-02
Publisher
JAPAN SOC BIOSCI BIOTECHN AGROCHEM
Citation
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY; Vol.74 2; 322-328
Keywords
Artemisia sacrorum LedebAMP-activated protein kinaseHepG2 cells
Abstract
Artemisia sacrorum Ledeb. (Compositae) (ASL) is a traditional Chinese medicine used to treat different hepatic diseases. However, a hypolipidemic effect of ASL on fatty liver disease has not been reported. Therefore, we investigated whether 95% ethanol eluate (EE), an active part of ASL, would attenuate hepatic lipid accumulation in human HepG2 cells by activating AMP-activated protein kinase (AMPK). Significant decreases in triglyceride levels and increases in AMPK and acetyl-CoA carboxylase (ACC) phosphorylation were observed when the cells were treated with 95% EE. EE down-regulated the lipogenesis gene expression of sterol regulatory element-binding protein 1c (SREBP1c) and its target genes, such as fatty acid synthase (FAS) and stearoyl-CoA desaturase 1 (SCD1), in a time- and dose-dependent manner. In contrast, the lipolytic gene expression of peroxisome proliferator-activated receptor alpha (PPAR-alpha) and CD36 increased in a time- and dose-dependent manner. These effects were abolished by pretreatment with compound C, an AMPK inhibitor. However, there were no differences in the gene expression of SREBP2, low density lipoprotein receptor (LDLR), hydroxymethyl glutaryl CoA reductase (HMG-CoA), or glucose transporter 2 (GLUT2). At the same time, 95% EE significantly increased the gene expression of acyl CoA oxidase (ACOX) in a time- and dose-dependent manner. Thus, AMPK mediated 95% EE induced suppression of SREBP1c and activation of PPAR-a respectively. These finding indicate that 95% EE attenuates hepatic lipid accumulation through AMPK activation and may be active in the prevention of serious diseases such as fatty liver, obesity, and type-2 diabetic mellitus.
ISSN
0916-8451
Language
English
URI
http://hdl.handle.net/10371/77456
DOI
https://doi.org/10.1271/bbb.90651
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse