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Differential diagnosis of Taenia asiatica using multiplex PCR

Cited 46 time in Web of Science Cited 52 time in Scopus
Authors
Jeon, Hyeong-Kyu; Chai, Jong-Yil; Kong, Yoon; Insisiengmay, Bounnaloth; Eom, Keeseon S.; Rim, Han-Jong; Waikagul, Jitra
Issue Date
2009-02
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
EXPERIMENTAL PARASITOLOGY; Vol.121 2; 151-156
Keywords
Taenia asiaticaT. soliumMultiplex PCRT. saginataDifferential diagnosis
Abstract
Taenia asiatica and T. saginata are frequently confused tapeworms due to their morphological similarities and sympatric distribution in Asian regions. To resolve this problem, a high-resolution multiplex PCR assay was developed to distinguish T. asiatica infections from infection with other human Taenia tapeworms. For molecular characterization, the species specificity of all materials used was confirmed by sequencing of the cox1 gene. Fifty-two samples were analyzed in this study, comprising 20 samples of T. asiatica genomic DNA from China, Korea, and the Philippines; 24 samples of T. saginata from Belgium, Chile, China, Ethiopia, France, Indonesia, Korea, Laos, the Philippines, Poland, Taiwan, Thailand, and Switzerland; and 10 samples of T. solium from Cape Verde, China, Honduras, and Korea. The diagnostic quality of the results obtained using PCR and species-specific primers designed from valine tRNA and NADH genes was equal to that based on the nucleotide sequencing of the cox1 gene. Using oligonucleotide primers Ta4978F, Ts5058F, Tso7421F, and Rev7915, the multiplex PCR assay was useful for the differentially diagnosing T. asiatica, T saginata, and T solium based on 706-, 629, and 474-bp bands. (C) 2008 Published by Elsevier Inc.
ISSN
0014-4894
Language
English
URI
http://hdl.handle.net/10371/77544
DOI
https://doi.org/10.1016/j.exppara.2008.10.014
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College of Medicine/School of Medicine (의과대학/대학원)Parasitology and Tropical Medicine (기생충학전공)Journal Papers (저널논문_기생충학전공)
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