Browse

The Antigenicity of Ex Vivo Cultivated Human Corneal Limbal Epithelial and Stromal Cells: Temporal Changes In Vitro

Cited 5 time in Web of Science Cited 4 time in Scopus
Authors
Oh, Joo Youn; Ko, Jung Hwa; Lee, Hyun Ju; Kim, Mee Kum; Wee, Won Ryang; Lee, Jin Hak
Issue Date
2010-11
Publisher
LIPPINCOTT WILLIAMS & WILKINS
Citation
CORNEA; Vol.29 11; 1302-1307
Keywords
corneal stromal celllimbal epithelial cellhuman leukocyte antigencostimulatory moleculecultivation
Abstract
Purpose: To investigate the effect of ex vivo culture on the expression of human leukocyte antigen (HLA) in human corneal limbal epithelial cells (LECs), and to evaluate the expression of HLA and costimulatory molecules on human corneal limbal stromal cells (LSCs). Methods: The expression of HLA-ABC and HLA-DR were evaluated using flow cytometry on cultured human LECs and LSCs after serial passage and compared with that on freshly isolated cells. Additionally, the expression of costimulatory molecules CD80, CD86, CD40, and immunoregulatory molecule HLA-G were investigated on LSCs with or without an addition of interferon (IFN) gamma (250 U/mL, 4 days). Results: HLA-ABC and HLA-DR were expressed in 99.12 +/- 1.02% and 25.86 +/- 4.34% of freshly isolated LECs, respectively. After culture, the HLA-DR-expressing cells significantly decreased with serial passage, whereas the percentage of HLA-ABC-expressing cells did not change. More than 90% of LSCs expressed HLA-ABC and HLA-G, whereas the expression of HLA-DR, CD80, CD86, and CD40 was negligible. This expression profile did not change after serial passage. However, the treatment with IFN-gamma induced a significant increase in HLA-DR (0.17% vs. 17.68%) and CD40 (0.19% vs. 13.19%) expression on LSCs. Conclusions: The immunogenicity of LECs can be lowered after ex vivo cultivation, whereas LSCs can be immunogenic in the presence of IFN-gamma.
ISSN
0277-3740
Language
English
URI
http://hdl.handle.net/10371/77814
DOI
https://doi.org/10.1097/ICO.0b013e3181e3f01e
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Ophthalmology (안과학전공)Journal Papers (저널논문_안과학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse