Browse

Array CGH를 이용한 DNA 복제 수 변화에 대한 분석에 따른 유방암의 예후 인자에 대한 후보 유전자로서의 COL18A1
COL18A1 as the Candidate Gene for the Prognostic Marker of Breast Cancer According to the Analysis of the DNA Copy Number Variation by Array CGH

DC Field Value Language
dc.contributor.author황기태-
dc.contributor.author정중기-
dc.contributor.author정인목-
dc.contributor.author허승철-
dc.contributor.author안영준-
dc.contributor.author안혜성-
dc.contributor.author장미수-
dc.contributor.author김정아-
dc.contributor.author한원식-
dc.contributor.author노동영-
dc.date.accessioned2012-06-29T06:28:00Z-
dc.date.available2012-06-29T06:28:00Z-
dc.date.issued2010-03-
dc.identifier.citationJOURNAL OF BREAST CANCER; Vol.13 1; 37-45ko_KR
dc.identifier.issn1738-6756-
dc.identifier.urihttp://hdl.handle.net/10371/77960-
dc.description.abstractPurpose: We tried to select and validate the candidate gene for the prognostic marker of breast cancer by comparing the analysis of copy number variation (CNV) between normal breast tissues and breast cancer tissues by performing array comparative genomic hybridization (CGH) Methods: Array CGH was performed with using the fresh frozen tissues of 77 breast cancer patients. We selected the clones with more than a 20% frequency of gain or loss, and the clones with gain or loss in more than 2 consecutive clones We finally selected the clones that were statistically significant on the survival analysis. We searched for the candidate gene that belonged to the candidate clones and we selected the final candidate gene that is assumed to be most related to the carcinogenesis of breast cancer by searching for information of the individual gene. We performed RT-PCR to validate the RNA expression of the final candidate gene with using the breast tissues of another 20 breast cancer patients. Results: Eleven (10 in the gain group and 1 in the loss group) clones were finally selected as candidate clones. The significant CNVs with gain were found in the regions of 1q23 1, 1q41, 1q44, 5p15.33, 8q21 3, 15q26.3, 17q12 and 21q22 3 and the significant CNV with loss was found in 14q32 33. COL18A1 (21q22.3) was selected as the final candidate gene and the RT-PCR results revealed that the expression of COL18A1 was up-regulated in the cancer tissues of 18 of the other 20 (90%) breast cancer patients. Conclusion: We selected COL18A1 (21q22 3) as the candidate gene for the prognostic marker of breast cancer by comparing the analysis of CNVs from the array CGH. The RNA of COL18A1 was over-expressed in breast cancer tissue, as determined by RT-PCR.ko_KR
dc.language.isokoko_KR
dc.publisherKOREAN BREAST CANCER SOCko_KR
dc.subjectBreast neoplasmsko_KR
dc.subjectMicroarray analysisko_KR
dc.subjectGene amplificationko_KR
dc.subjectCollagen type XVIIIko_KR
dc.subject유방 신생물ko_KR
dc.subject콜라겐 type XVIIIko_KR
dc.subject유전자 증폭ko_KR
dc.subject미세배열 분석ko_KR
dc.titleArray CGH를 이용한 DNA 복제 수 변화에 대한 분석에 따른 유방암의 예후 인자에 대한 후보 유전자로서의 COL18A1ko_KR
dc.title.alternativeCOL18A1 as the Candidate Gene for the Prognostic Marker of Breast Cancer According to the Analysis of the DNA Copy Number Variation by Array CGHko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthorHwang, Ki-Tae-
dc.contributor.AlternativeAuthorChung, Jung Kee-
dc.contributor.AlternativeAuthorJung, In Mok-
dc.contributor.AlternativeAuthorHeo, Seung Chul-
dc.contributor.AlternativeAuthorAhn, Hye Seong-
dc.contributor.AlternativeAuthorKim, Jeong-Ah-
dc.contributor.AlternativeAuthorNoh, Dong-Young-
dc.contributor.AlternativeAuthorHan, Wonshik-
dc.contributor.AlternativeAuthorChang, Mee Soo-
dc.contributor.AlternativeAuthorAhn, Young Joon-
dc.identifier.doi10.4048/jbc.2010.13.1.37-
dc.citation.journaltitleJOURNAL OF BREAST CANCER-
dc.description.citedreferenceThomassen M, 2009, BREAST CANCER RES TR, V113, P239, DOI 10.1007/s10549-008-9927-2-
dc.description.citedreferenceHwang KT, 2008, INT J CANCER, V123, P1807, DOI 10.1002/ijc.23672-
dc.description.citedreferenceTan DSR, 2008, PATHOBIOLOGY, V75, P63, DOI 10.1159/000123844-
dc.description.citedreferenceSotiriou C, 2007, NAT REV CANCER, V7, P545, DOI 10.1038/nrc2173-
dc.description.citedreferenceBalasubramanian SP, 2007, BMC CANCER, V7, DOI 10.1186/1471-2407-7-107-
dc.description.citedreferenceChand N, 2007, INT J AD HOC UBIQ CO, V2, P58, DOI 10.1504/IJAHUC.2007.011604-
dc.description.citedreferenceWoo IS, 2006, INT J CANCER, V119, P2901, DOI 10.1002/ijc.22216-
dc.description.citedreferenceLourenco GJ, 2006, BREAST CANCER RES TR, V100, P335, DOI 10.1007/s10549-006-9259-z-
dc.description.citedreferenceKim MY, 2006, GASTROENTEROLOGY, V131, P1913, DOI 10.1053/j.gastro.2006.10.021-
dc.description.citedreferenceBrzezianska E, 2006, MUTAT RES-FUND MOL M, V599, P26, DOI 10.1016/j.mrfmmm.2005.12.013-
dc.description.citedreferenceDang CX, 2006, J GASTROEN HEPATOL, V21, P850, DOI 10.1111/j.1440-1746.2006.04074.x-
dc.description.citedreferenceHan W, 2006, BMC CANCER, V6, DOI 10.1186/1471-2407-6-92-
dc.description.citedreferencevan Beers EH, 2006, BREAST CANCER RES, V8, DOI 10.1186/bcr1510-
dc.description.citedreferenceHu TH, 2005, MODERN PATHOL, V18, P663, DOI 10.1038/modpathol.3800336-
dc.description.citedreferenceTokusashi Y, 2005, INT J CANCER, V114, P39, DOI 10.1002/ijc.20685-
dc.description.citedreferenceNessling M, 2005, CANCER RES, V65, P439-
dc.description.citedreferenceNaylor TL, 2005, BREAST CANCER RES, V7, pR1186, DOI 10.1186/bcr1356-
dc.description.citedreferenceDavies JJ, 2005, CHROMOSOME RES, V13, P237, DOI 10.1007/s10577-005-2168-x-
dc.description.citedreferenceSWIDZINSKA E, 2005, ROSZ AKAD MED BIALYM, V50, P197-
dc.description.citedreferenceIizasa T, 2004, CLIN CANCER RES, V10, P5361-
dc.description.citedreferenceKikuchi E, 2004, CLIN CANCER RES, V10, P1835-
dc.description.citedreferenceRennstam K, 2003, CANCER RES, V63, P8861-
dc.description.citedreferenceBeck MT, 2003, CANCER RES, V63, P3598-
dc.description.citedreferenceChebil G, 2003, ACTA ONCOL, V42, P43-
dc.description.citedreferenceKIM HS, 2003, J BREAST CANCERER, V6, P58-
dc.description.citedreferenceLiu D, 2002, BRIT J CANCER, V87, P423, DOI 10.1038/sj.bjc.6600456-
dc.description.citedreferenceHAN W, 2002, J BREAST CANCERER, V2, P284-
dc.description.citedreferenceNakatani K, 1999, J BIOL CHEM, V274, P21528-
dc.description.tc0-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Surgery (외과학전공)Journal Papers (저널논문_외과학전공)
Files in This Item:
There are no files associated with this item.
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse