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Cytokine secretion by human mesenchymal stem cells cocultured with damaged corneal epithelial cells

Cited 42 time in Web of Science Cited 43 time in Scopus
Authors
Oh, Joo Youn; Kim, Mee Kum; Shin, Mi Sun; Wee, Won Ryang; Lee, Jin Hak
Issue Date
2009-04
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation
CYTOKINE; Vol.46(1); 100-103
Keywords
AngiogenesisMesenchymal stem cellInflammationCorneaCytokine
Abstract
We have previously shown that mesenchymal stem cells (MSCs) reduced corneal inflammation and neovascularization in chemically-burned rat corneas in part through paracrine action. In order to identify the molecule(s) involved, we cocultured human MSCs in the following conditions, and examined the alterations in the cytokine secretion profile; (1) human peripheral blood mononuclear cells (hPBMCs), (2) chemically-damaged human corneal epithelial cells (hCECs), (3) hPBMCs/hCECs, (4) hMSCs, (5) hMSCs/hPBMCs, (6) hMSCs/hCECs, (7) hMSCs/hPBMCs/hCECs. We found that the levels of interleukin (IL)-6 and vascular endothelial growth factor (VEGF) by hMSCs markedly increased for hMSC/hCEC cocultures, and this elevation was further remarkable by the addition of hPBMCs. The hMSCs constitutively expressed transforming growth factor (TGF)-beta 1, matrix metalloproteinase (MMP)-2, and thrombospondin-1. The secretion of MMP-9 by hCECs was significantly suppressed by hMSCs. Tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, and IL-10 were not detectable in any cultures. The data presented herein provide the candidate molecules mediating the MSC-mediated modulation of inflammation and angiogenesis in cornea. (C) 2009 Elsevier Ltd. All rights reserved.
ISSN
1043-4666
Language
English
URI
http://hdl.handle.net/10371/78018
DOI
https://doi.org/10.1016/j.cyto.2008.12.011
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College of Medicine/School of Medicine (의과대학/대학원)Ophthalmology (안과학전공)Journal Papers (저널논문_안과학전공)
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