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The Anti-inflammatory Effect of Subconjunctival Bevacizumab on Chemically Burned Rat Corneas

Cited 34 time in Web of Science Cited 41 time in Scopus
Authors
Oh, Joo Youn; Kim, Mee Kum; Shin, Mi Sun; Lee, Hyun Ju; Wee, Won Ryang; Lee, Jin Hak
Issue Date
2009
Publisher
INFORMA HEALTHCARE
Citation
CURRENT EYE RESEARCH; Vol.34 2; 85-91
Keywords
angiogenesisinflammationepitheliumbevacizumabcornea
Abstract
Purpose: To investigate the effect of bevacizumab in a model of corneal inflammation. Materials and Methods: Epithelium from the cornea and limbus was completely removed using 100% alcohol in rats. Bevacizumab (1.25 mg/0.05 ml) or normal saline was subconjunctivally injected. One week later, corneas were examined and subjected to hematoxylin-eosin and immunofluorescent staining for VEGF. Expression of IL-2, IFN-, IL-6, and TGF- 1 were analyzed by ELISA. Results: Bevacizumab showed a borderline reduction in corneal neovascularization (11.0 4.8% and 18.0 10.0% in the bevacizumab and control groups, respectively; p = 0.054), while the extent of epithelialization was not affected (5.0 3.4% and 6.1 5.2% in the bevacizumab and control groups, respectively; p = 0.715). The infiltration of inflammatory cells was reduced (99.3 22.3 cells/ 400 in the bevacizumab-injected corneas and 182.3 58.0 cells/ 400 in the controls; p = 0.013). The levels of IL-2, IFN-, and IL-6 were decreased in the rats with the bevacizumab injections (44 6 and 79 9 pg/ml for IL-2 in the bevacizumab-injected group and control, respectively, p = 0.025; 45 5 and 67 6 pg/ml for IFN- in the bevacizumab-injected group and controls, respectively, p = 0.043; 45 6 and 75 8 pg/ml for IL-6 in the bevacizumab-injected group and controls, respectively, p = 0.030). Conclusions: Bavacizumab showed a reduction in inflammatory cell infiltration and cytokines in chemically burned rat corneas.
ISSN
0271-3683
Language
English
URI
http://hdl.handle.net/10371/78052
DOI
https://doi.org/10.1080/02713680802607740
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College of Medicine/School of Medicine (의과대학/대학원)Ophthalmology (안과학전공)Journal Papers (저널논문_안과학전공)
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