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Identification of alpha-Gal and non-Gal Epitopes in Pig Corneal Endothelial Cells and Keratocytes by Using Mass Spectrometry

Cited 25 time in Web of Science Cited 26 time in Scopus
Authors
Kim, Yun-Gon; Oh, Joo Youn; Gil, Geun-Cheol; Kim, Mee Kum; Lee, Sukmook; Wee, Won Ryang; Kim, Byung-Gee; Lee, Hyun Ju; Ko, Jung Hwa
Issue Date
2009
Publisher
INFORMA HEALTHCARE
Citation
CURRENT EYE RESEARCH; Vol.34 10; 877-895
Keywords
alpha-galactoseCorneal endothelial cellsXenotransplantationPigNon-Gal antigensMass spectrometryKeratocytes
Abstract
Purpose: To investigate the expression of alpha-Gal or unidentified non-Gal antigens in pig corneal endothelial cells and keratocytes, we performed the qualitative and quantitative analysis by using mass spectrometry. Methods: The N-glycans from common adult pig corneal endothelial cells and keratocytes cultured in vitro were directly analyzed by using mass spectrometric approaches. In addition, immuno chemical staining was added to confirm the non-Gal antigen expression in pig corneal cells. Results: Totally, 34 of the sialylated N-glycans from pig corneal endothelial cells and 27 from pig keratocytes were identified and observed to contain nonhuman sialic acid, NeuGc as well as NeuAc. In addition, we were able to detect 25 of alpha-galactosylated N-glycan structures (22.2% of total) from the pig corneal endothelial cells and 18 of that (17.5% of total) from the pig keratocytes by using mass spectrometric approaches. On immunofluorescent staining, the expression of sialylated glycans was also observed. Conclusions: As well as alpha-Gal epitopes, several promising non-Gal antigens were widely expressed on both pig corneal endothelial cells and keratocytes. The detailed structural information of the alpha-Gal and non-Gal epitopes would be a tremendous value to develop a new strategy for the successful corneal xenotransplantation in future.
ISSN
0271-3683
Language
English
URI
http://hdl.handle.net/10371/78053
DOI
https://doi.org/10.3109/02713680903184243
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College of Medicine/School of Medicine (의과대학/대학원)Ophthalmology (안과학전공)Journal Papers (저널논문_안과학전공)
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