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Flavonoids inhibit the AU-rich element binding of HuC

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Authors
Kwak, Hojoong; Jeong, Kyung-Chae; Chae, Min-Ju; Kim, Soo-Youl; Park, Woong-Yang
Issue Date
2009-01-31
Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
Citation
BMB REPORTS; Vol.42 1; 41-46
Keywords
AU-rich elementNatural compoundsHuCElectrophoretic mobility shift assayFlavonoids
Abstract
Post-transcriptional regulation of mRNA stability by Hu proteins is an important mechanism for tumorigenesis. We focused on the molecular interactions between the HuC protein and AU-rich elements (AREs) to find chemical inhibitors of RNA-protein interactions using RNA electrophoretic mobility shift assay with non-radioactive probes. Screening of 52 natural compounds identified 14 candidate compounds that displayed potent inhibitory activity. Six (quercetin, myricetin, (-)-epigallocatechin gallate, ellagic acid, (-)-epicatechin gallate, and rhamnetin) were categorized as phytochemicals, and their IC(50) values were low (0.2-1.8 mu M).
ISSN
1976-6696
Language
English
URI
http://hdl.handle.net/10371/78160
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)Journal Papers (저널논문_의학과)
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