Publications
Detailed Information
Chemical inhibitors destabilize HuR binding to the AU-rich element of TNF-alpha mRNA
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chae, Min-Ju | - |
dc.contributor.author | Sung, Hye Youn | - |
dc.contributor.author | Kim, Eun-Hye | - |
dc.contributor.author | Lee, Mira | - |
dc.contributor.author | Chae, Chong Hak | - |
dc.contributor.author | Park, Woong-Yang | - |
dc.contributor.author | Kim, Sunwoo | - |
dc.contributor.author | Kwak, Hojoong | - |
dc.date.accessioned | 2012-07-03T02:22:18Z | - |
dc.date.available | 2012-07-03T02:22:18Z | - |
dc.date.issued | 2009-11-30 | - |
dc.identifier.citation | EXPERIMENTAL AND MOLECULAR MEDICINE; Vol.41 11; 824-831 | ko_KR |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://hdl.handle.net/10371/78194 | - |
dc.description.abstract | Hu protein R (HuR) binds to the AU-rich element (ARE) in the 3`UTR to stabilize TNF-alpha mRNA. Here, we identified chemical inhibitors of the interaction between HuR and the ARE of TNF-alpha mRNA using RNA electrophoretic mobility gel shift assay (EMSA) and filter binding assay. Of 179 chemicals screened, we identified three with a half-maximal inhibitory concentration (IC(50)) below 10 mu M. The IC(50) of quercetin, b-40, and b-41 were 1.4, 0.38, and 6.21 mu M, respectively, for binding of HuR protein to TNF-alpha mRNA. Quercetin and b-40 did not inhibit binding of tristetraprolin to the ARE of TNF-alpha mRNA. When LPS-treated RAW264.7 cells were treated with quercetin and b-40, we observed decreased stability of TNF-alpha mRNA and decreased levels of secreted TNF-alpha. From these results, we could find inhibitors for the TNF-alpha mRNA stability, which might be used advantageously for both the study for post-transcriptional regulation and the discovery of new anti-inflammation drugs. | ko_KR |
dc.description.sponsorship | This work was supported by a grant
from the Ministry of Education, Science and Technology, Korea (M20706000020-07M0600-02010, W.Y.P.), the BK21 program (W.Y.P.), and Seoul National University Hospital (W.Y.P.). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY | ko_KR |
dc.subject | anti-inflammatory agents | ko_KR |
dc.subject | ELAV-Iike protein 1 | ko_KR |
dc.subject | lipopolysaccharides | ko_KR |
dc.subject | tumor necrosis factor-alpha | ko_KR |
dc.subject | quercetin | ko_KR |
dc.subject | macrophages | ko_KR |
dc.title | Chemical inhibitors destabilize HuR binding to the AU-rich element of TNF-alpha mRNA | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 채민주 | - |
dc.contributor.AlternativeAuthor | 성혜윤 | - |
dc.contributor.AlternativeAuthor | 김은혜 | - |
dc.contributor.AlternativeAuthor | 이미라 | - |
dc.contributor.AlternativeAuthor | 곽호중 | - |
dc.contributor.AlternativeAuthor | 채총학 | - |
dc.contributor.AlternativeAuthor | 김선우 | - |
dc.contributor.AlternativeAuthor | 박웅양 | - |
dc.identifier.doi | 10.3858/emm.2009.41.11.088 | - |
dc.citation.journaltitle | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.description.citedreference | Kwak H, 2009, BMB REP, V42, P41 | - |
dc.description.citedreference | Grattendick KJ, 2008, INT IMMUNOPHARMACOL, V8, P679, DOI 10.1016/j.intimp.2008.01.013 | - |
dc.description.citedreference | Meisner NC, 2007, NAT CHEM BIOL, V3, P508, DOI 10.1038/nchembio.2007.14 | - |
dc.description.citedreference | Seko Y, 2006, AUTOIMMUN REV, V5, P299, DOI 10.1016/j.autrev.2005.10.013 | - |
dc.description.citedreference | Jung KC, 2006, LEUKEMIA, V20, P122, DOI 10.1038/sj.leu.2404022 | - |
dc.description.citedreference | Fan J, 2005, EUR RESPIR J, V26, P933, DOI 10.1183/09031936.05.00120204 | - |
dc.description.citedreference | McMullen MR, 2003, J BIOL CHEM, V278, P38333, DOI 10.1074/jbc.M304566200 | - |
dc.description.citedreference | Kumar S, 2003, NAT REV DRUG DISCOV, V2, P717, DOI 10.1038/nrd1177 | - |
dc.description.citedreference | Mazan-Mamczarz K, 2003, P NATL ACAD SCI USA, V100, P8354, DOI 10.1073/pnas.1432104100 | - |
dc.description.citedreference | Zhang T, 2002, BIOCHEM SOC T, V30, P952 | - |
dc.description.citedreference | Hollams EM, 2002, NEUROCHEM RES, V27, P957, DOI 10.1023/A:1020992418511 | - |
dc.description.citedreference | Kang MJ, 2002, BIOCHEM BIOPH RES CO, V293, P617, DOI 10.1016/S0006-291X(02)00264-4 | - |
dc.description.citedreference | Dean JLE, 2001, MOL CELL BIOL, V21, P721, DOI 10.1128/MCB.21.3.721-730.2001 | - |
dc.description.citedreference | Brennan CM, 2001, CELL MOL LIFE SCI, V58, P266, DOI 10.1007/PL00000854 | - |
dc.description.citedreference | Wang WG, 2000, MOL CELL BIOL, V20, P760, DOI 10.1128/MCB.20.3.760-769.2000 | - |
dc.description.citedreference | Lai WS, 1999, MOL CELL BIOL, V19, P4311 | - |
dc.description.citedreference | Fan XHC, 1998, EMBO J, V17, P3448, DOI 10.1093/emboj/17.12.3448 | - |
dc.description.citedreference | Levy NS, 1998, J BIOL CHEM, V273, P6417, DOI 10.1074/jbc.273.11.6417 | - |
dc.description.citedreference | Ma WJ, 1996, J BIOL CHEM, V271, P8144 | - |
dc.description.citedreference | BEUTLER B, 1989, ANNU REV IMMUNOL, V7, P625, DOI 10.1146/annurev.iy.07.040189.003205 | - |
dc.description.tc | 8 | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.