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Copper-GHK increases integrin expression and p63 positivity by keratinocytes

Cited 23 time in Web of Science Cited 22 time in Scopus
Authors
Kang, Youn-A; Choi, Hye-Ryung; Na, Jung-Im; Huh, Chang-Hun; Youn, Sang-Woong; Park, Kyoung-Chan; Kim, Kyu-Han; Kim, Min-Ji
Issue Date
2009-05
Publisher
SPRINGER
Citation
ARCHIVES OF DERMATOLOGICAL RESEARCH; Vol.301, no.4; 301-306
Keywords
PeptideSkin equivalentsIntegrinStem cellsp63Copper-GHKKeratinocytes
Abstract
Glycyl-l-histidyl-l-lysyl (GHK) possesses a high affinity for copper(II) ions, with which it spontaneously forms a complex (copper-GHK). It is well known that copper-GHK plays a physiological role in the process of wound healing and tissue repair by stimulating collagen synthesis in fibroblasts. This study was conducted to investigate the effects of copper-GHK on keratinocytes. Proliferative effects were analyzed and hematoxylin and eosin staining and immunohistochemistry were conducted to evaluate the effects of copper-GHK in skin equivalent (SE) models. In addition, western blotting was performed. In monolayer cultured keratinocytes, copper-GHK increased the proliferation of keratinocytes. When the SE models were evaluated, basal cells became cuboidal when copper-GHK was added. Immunohistochemical analysis revealed that copper-GHK increased proliferating cell nuclear antigen (PCNA) and p63 positivity. Furthermore, the expression of integrin alpha 6 and beta 1 increased in SE models, and these results were confirmed by Western blotting. The results of this study indicate that treatment with copper-GHK may increase the proliferative potential of basal keratinocytes by modulating the expression of integrins, p63 and PCNA. In addition, increased levels of p63, a putative stem cell marker of the skin, suggests that copper-GHK promotes the survival of basal stem cells in the skin.
ISSN
0340-3696
Language
English
URI
http://hdl.handle.net/10371/78206
DOI
https://doi.org/10.1007/s00403-009-0942-x
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College of Medicine/School of Medicine (의과대학/대학원)Dermatology (피부과학전공)Journal Papers (저널논문_피부과학전공)
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