Publications
Detailed Information
NTA center dot Ni2+-Functionalized Quantum Dots for VAMP2 Labeling in Live Cells
Cited 0 time in
Web of Science
Cited 0 time in Scopus
- Authors
- Issue Date
- 2010-06-20
- Publisher
- KOREAN CHEMICAL SOC
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY; Vol.31 6; 1474-1478
- Keywords
- Quantum dot conjugates ; Imaging probes ; Protein tracking ; Labeling
- Abstract
- An efficient method for labeling individual proteins in live cells is required for investigations into biological mechanisms and cellular processes. Here we describe the preparation of small quantum dots (QDs) that target membrane surface proteins bearing a hexahistidine-tag (His(6)-tag) via specific binding to an nitrilotriacetic acid complex of nickel(II) (NTA center dot Ni2+) on the QD surfaces. We showed that the NTA center dot Ni2+-QDs bound to His-tag functionalized beads as a cellular mimic with high specificity and that QDs successfully targeted His(6)-tagged vesicle-associated membrane proteins (VMAP) on cell surfaces. This strategy provides an efficient approach to monitoring synaptic protein dynamics in spatially restricted and confined biological environments.
- ISSN
- 0253-2964
- Language
- English
- Files in This Item:
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.