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Smart Magnetic Fluorescent Nanoparticle Imaging Probes to Monitor MicroRNAs
Cited 68 time in
Web of Science
Cited 77 time in Scopus
- Authors
- Issue Date
- 2010-01-04
- Publisher
- WILEY-V C H VERLAG GMBH
- Citation
- SMALL; Vol.6 1; 81-88
- Keywords
- fluorescence ; imaging ; nanoparticles ; RNA ; molecular beacons
- Abstract
- An imaging system that can be used to evaluate the expression levels of microRNAs during neuronal development can provide noninvasive information for investigating a variety of biological phenomena related to microRNAs (miRNAs, miRs). Herein, the development of a novel imaging platform to monitor intracellular miR124a during neuronal differentiation is reported using rhodamine-coated cobalt ferrite magnetic fluorescent (MF) nanoparticles linked to a quenching molecular system containing an miR124a binding sequence (MF-miR124a beacon). During neuronal differentiation, in vitro fluorescence signals of the MF-miR124a beacon are significantly increased under conditions where miR124a is highly expressed, and dramatically return to the original quenched fluorescence after anti-miR124a treatment. In vivo fluorescence images show enhanced fluorescence signals in mice with P19 cells within a poly-L-lactic acid scaffold after induction of neuronal differentiation. In addition, magnetic resonance (MR) images provide in vivo tracking of cells containing the MF-miR124a beacon. These studies represent the first step toward the use of nanotechnological imaging of mature miRNA, and this technique could be used for cellular tracking with a MR imaging system as well as for simultaneous monitoring of the miRNA expression pattern in vivo.
- ISSN
- 1613-6810
- Language
- English
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