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Smart Magnetic Fluorescent Nanoparticle Imaging Probes to Monitor MicroRNAs

Cited 61 time in Web of Science Cited 68 time in Scopus
Authors
Hwang, Do Won; Song, In Chan; Lee, Dong Soo; Kim, Soonhag
Issue Date
2010-01-04
Publisher
WILEY-V C H VERLAG GMBH
Citation
SMALL; Vol.6 1; 81-88
Keywords
fluorescenceimagingnanoparticlesRNAmolecular beacons
Abstract
An imaging system that can be used to evaluate the expression levels of microRNAs during neuronal development can provide noninvasive information for investigating a variety of biological phenomena related to microRNAs (miRNAs, miRs). Herein, the development of a novel imaging platform to monitor intracellular miR124a during neuronal differentiation is reported using rhodamine-coated cobalt ferrite magnetic fluorescent (MF) nanoparticles linked to a quenching molecular system containing an miR124a binding sequence (MF-miR124a beacon). During neuronal differentiation, in vitro fluorescence signals of the MF-miR124a beacon are significantly increased under conditions where miR124a is highly expressed, and dramatically return to the original quenched fluorescence after anti-miR124a treatment. In vivo fluorescence images show enhanced fluorescence signals in mice with P19 cells within a poly-L-lactic acid scaffold after induction of neuronal differentiation. In addition, magnetic resonance (MR) images provide in vivo tracking of cells containing the MF-miR124a beacon. These studies represent the first step toward the use of nanotechnological imaging of mature miRNA, and this technique could be used for cellular tracking with a MR imaging system as well as for simultaneous monitoring of the miRNA expression pattern in vivo.
ISSN
1613-6810
Language
English
URI
http://hdl.handle.net/10371/78428
DOI
https://doi.org/10.1002/smll.200901262
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College of Medicine/School of Medicine (의과대학/대학원)Radiology (영상의학전공)Journal Papers (저널논문_영상의학전공)
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