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Effect of Serum and Growth Factors on Chondrogenic Differentiation of Synovium-Derived Stromal Cells

Cited 26 time in Web of Science Cited 29 time in Scopus
Authors
Lee, Sahnghoon; Kim, Ji Hyun; Jo, Chris Hyunchul; Seong, Sang Cheol; Lee, Myung Chul; Lee, Jae Chul
Issue Date
2009-11
Publisher
MARY ANN LIEBERT INC
Citation
TISSUE ENGINEERING PART A; Vol.15(11); 3401-3415
Abstract
We used fetal bovine serum (FBS) and different growth factors to investigate their potential for inducing chondrogenic differentiation of synovium-derived stromal cells. Human synovium was harvested from patients suffering from osteoarthritis and expanded in monolayer. To evaluate the effect of serum and growth factors on chondrogenic differentiation, 10 ng/mL of transforming growth factor-beta 1 (TGF-beta 1), 100 ng/mL of bone morphogenic protein-2 (BMP-2), 100 ng/mL of insulin-like growth factor-1, 20 ng/mL of basic fibroblast growth factor (bFGF), and 10% FBS were added to the chemically defined chondrogenic medium singly or in combination during pellet culture for 21 days. The cell size and weight, glycosaminoglycan content, histology, and cartilage matrix-associated genes expression were analyzed. TGF-beta 1 alone and TGF-beta 1 + BMP-2 induced chondrogenic differentiation of synovium-derived stromal cells and synthesized cartilage-like matrix confirmed by histological analysis and immunohistochemistry. FBS, BMP-2, insulin-like growth factor-1, and bFGF as a single factor or other combinations except for TGF-beta 1 + BMP-2 hardly induced chondrogenesis. Chondrogenic differentiation appeared to be inhibited when bFGF or the serum was added to the chondrogenic medium during pellet culture. The results of this study demonstrate the negative or positive role of serum and growth factors on chondrogenic differentiation of synovium-derived stromal cells.
ISSN
1937-3341
Language
English
URI
http://hdl.handle.net/10371/78445
DOI
https://doi.org/10.1089/ten.tea.2008.0466
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College of Medicine/School of Medicine (의과대학/대학원)Orthopedic Surgery (정형외과학전공)Journal Papers (저널논문_정형외과학전공)
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