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Dexamethasone treatment inhibits VEGF production via suppression of STAT3 in a head and neck cancer cell line

Cited 14 time in Web of Science Cited 0 time in Scopus
Authors
Shim, Seon-Hui; Hah, J. Hun; Hwang, So-Yeon; Heo, Dae Seog; Sung, Myung-Whun
Issue Date
2010-04
Publisher
SPANDIDOS PUBL LTD
Citation
ONCOLOGY REPORTS; Vol.23 4; 1139-1143
Keywords
dexamethasonesignal transducer and activator of transcription 3head and neck squamous cell carcinomavascular endothelial growth factor
Abstract
Glucocorticoids (GCs) modulate the synthesis of many pro-inflammatory cytokines and influence multiple transduction pathways. GCs negatively or positively influence the transcription factors of their target genes. All of these transcription signals are closely connected to cancer survival or death. We investigated the action of dexamethasone (DEX) on head and neck cancer cell lines. When SNU-1041 and SNU-1076 were treated with DEX, the cell lines showed different patterns of responses. DEX inhibition of cell growth depended on concentration in SNU-1041, but not in SNU-1076. Furthermore, DEX Suppressed Vascular endothelial growth factor (VEGF) secretion from SNU-1041, but not from SNU-1076. We explored the mechanism that explains these distinct differences. After DEX treatment, the differences of NF-kappa B (p65), glucocorticoid receptor and p-AKT were not observed between the cell lines. However, phospho-signal transducer and activator of transcription 3 (STAT3) decreased in SNU-1041 only. Moreover, STAT3 inhibition using si-RNA Suppressed VEGF secretion. When STAT3 was overexpressed after DEX treatment, the level of VEGF in the culture media was restored. Taken together, we suggest that p-STAT3 can be a mediating factor which regulates VEGF secretion in the DEX treatment. Because the relationship between the three Molecules DEX, STAT3 and VEGF is scarcely known, our findings clarified one of the signaling pathways of DEX, which is often used in clinical conditions.
ISSN
1021-335X
Language
English
URI
http://hdl.handle.net/10371/78479
DOI
https://doi.org/10.3892/or_00000743
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College of Medicine/School of Medicine (의과대학/대학원)Otorhinolaryngology (이비인후과학전공)Journal Papers (저널논문_이비인후과학전공)
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